Leishmania mexicana amazonensis: Plasma membrane adenine nucleotide translocator and chemotaxis
| Autor: | Siegfried Detke, R. Elsabrouty |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Blotting
Western Leishmania mexicana Immunology Biology Article parasitic diseases Animals Nucleotide chemistry.chemical_classification Microscopy Confocal Negative chemotaxis Chemotaxis Adenine nucleotide translocator Cell Membrane fungi General Medicine biology.organism_classification Leishmania Immunohistochemistry ANT Cell biology Infectious Diseases Microscopy Fluorescence Biochemistry chemistry biology.protein Parasitology Mitochondrial ADP ATP Translocases Nucleoside |
| Zdroj: | Experimental Parasitology. 118:408-419 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2007.10.010 |
| Popis: | Leishmania cannot synthesize purines de novo and rely on their host to furnish these compounds. To accomplish this, they possess multiple purine nucleoside and nucleobase transporters. Subcellular fractionation, immunohistochemical localization with anti-adenine nucleotide translocator (ANT) antibodies and surface biotinylation show that the mitochondrial ANT is also present in the plasma membrane of both promastigotes and amastigotes. Leishmania, however, do not appear to rely on this transporter to supplement their purine or energy requirements via preformed ATP from its host. Rather, Leishmania appear to use the plasma membrane ANT as part of a chemotaxis response. ATP is a chemorepellant for Leishmania and cells treated with atractyloside, an inhibitor of ANT, no longer exhibit negative chemotaxis for this compound. |
| Databáze: | OpenAIRE |
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