ZnO Nano-Rod Devices for Intradermal Delivery and Immunization
| Autor: | Nayak, Tapas R, Wang, Hao, Pant, Aakansha, Zheng, Minrui, Junginger, Hans, Goh, Wei Jiang, Lee, Choon Keong, Zou, Shui, Alonso, Sylvie, Czarny, Bertrand, Storm, Gert, Sow, Chorng Haur, Lee, Chengkuo, Pastorin, Giorgia, Pharmaceutics, Afd Pharmaceutics |
|---|---|
| Přispěvatelé: | School of Materials Science & Engineering, Lee Kong Chian School of Medicine (LKCMedicine), Pharmaceutics, Afd Pharmaceutics |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
General Chemical Engineering Confocal intradermal delivery Nanotechnology Human skin 02 engineering and technology skin immunization Article ZnO Nano-rods lcsh:Chemistry 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine ZnO nano-rods Antigen General Materials Science Bradford protein assay biology technology industry and agriculture Penetration (firestop) Permeation 021001 nanoscience & nanotechnology Engineering::Materials [DRNTU] Ovalbumin lcsh:QD1-999 Drug delivery biology.protein Skin Immunization 0210 nano-technology Biomedical engineering |
| Zdroj: | Nanomaterials Nanomaterials; Volume 7; Issue 6; Pages: 147 Nanomaterials, Vol 7, Iss 6, p 147 (2017) Nanomaterials (Basel, Switzerland), 7(6). Multidisciplinary Digital Publishing Institute |
| ISSN: | 2079-4991 |
| Popis: | Intradermal delivery of antigens for vaccination is a very attractive approach since the skin provides a rich network of antigen presenting cells, which aid in stimulating an immune response. Numerous intradermal techniques have been developed to enhance penetration across the skin. However, these methods are invasive and/or affect the skin integrity. Hence, our group has devised zinc oxide (ZnO) nano-rods for non-destructive drug delivery. Chemical vapour deposition was used to fabricate aligned nano-rods on ZnO pre-coated silicon chips. The nano-rods’ length and diameter were found to depend on the temperature, time, quality of sputtered silicon chips, etc. Vertically aligned ZnO nano-rods with lengths of 30–35 µm and diameters of 200–300 nm were selected for in vitro human skin permeation studies using Franz cells with Albumin-fluorescein isothiocyanate (FITC) absorbed on the nano-rods. Fluorescence and confocal studies on the skin samples showed FITC penetration through the skin along the channels formed by the nano-rods. Bradford protein assay on the collected fluid samples indicated a significant quantity of Albumin-FITC in the first 12 h. Low antibody titres were observed with immunisation on Balb/c mice with ovalbumin (OVA) antigen coated on the nano-rod chips. Nonetheless, due to the reduced dimensions of the nano-rods, our device offers the additional advantage of excluding the simultaneous entrance of microbial pathogens. Taken together, these results showed that ZnO nano-rods hold the potential for a safe, non-invasive, and painless intradermal drug delivery. Published version |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|