Gut microbiota diversity predicts immune status in HIV-1 infection
| Autor: | Anders Sönnerborg, Hov, Babilonia Barqasho, Ujjwal Neogi, Jenny Svärd, Kajsa Noyan, Ekaterina Avershina, Marius Trøseid, Knut Rudi, Piotr Nowak, Kristian Holm, Jan Vesterbacka |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
DNA Bacterial Male Immunology Molecular Sequence Data Enzyme-Linked Immunosorbent Assay HIV Infections Gut flora Systemic inflammation DNA Ribosomal Cohort Studies Young Adult RNA Ribosomal 16S Adjuvant therapy Prevotella medicine Immunology and Allergy Humans Young adult Limulus Test Aged biology Monocyte Microbiota Sequence Analysis DNA Middle Aged biology.organism_classification medicine.disease Gastrointestinal Microbiome Infectious Diseases medicine.anatomical_structure Limulus amebocyte lysate Bacterial Translocation HIV-1 Dysbiosis Female medicine.symptom |
| Zdroj: | AIDS (London, England). 29(18) |
| ISSN: | 1473-5571 |
| Popis: | OBJECTIVE HIV-1 infection is characterized by altered intestinal barrier, gut microbiota dysbiosis, and systemic inflammation. We hypothesized that changes of the gut microbiota predict immune dysfunction and HIV-1 progression, and that antiretroviral therapy (ART) partially restores the microbiota composition. DESIGN An observational study including 28 viremic patients, three elite controllers, and nine uninfected controls. Blood and stool samples were collected at baseline and for 19 individuals at follow-up (median 10 months) during ART. METHODS Microbiota composition was determined by 16S rRNA sequencing (Illumina MiSeq). Soluble markers of microbial translocation and monocyte activation were analyzed by Limulus Amebocyte Lysate assay or ELISA. RESULTS Several alpha-diversity measures, including number of observed bacterial species and Shannon index, were significantly lower in viremic patients compared to controls. The alpha diversity correlated with CD4 T-cell counts and inversely with markers of microbial translocation and monocyte activation. In multivariate linear regression, for every age and sex-adjusted increase in the number of bacterial species, the CD4 T-cell count increased with 0.88 (95% confidence interval 0.35-1.41) cells/μl (P = 0.002). After introduction of ART, microbiota alterations persisted with further reduction in alpha diversity. The microbiota composition at the genus level was profoundly altered in viremic patients, both at baseline and after ART, with Prevotella reduced during ART (P < 0.007). CONCLUSIONS Gut microbiota alterations are closely associated with immune dysfunction in HIV-1 patients, and these changes persist during short-term ART. Our data implicate that re-shaping the microbiota may be an adjuvant therapy in patients commencing successful ART. |
| Databáze: | OpenAIRE |
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