Biological parameters, immune enzymes, and histological alterations in the livers of grass carp infected with Aeromonas hydrophila
| Autor: | Yuan Fang, Yubang Shen, Xin-Zhan Meng, Rongquan Wang, Jiale Li, Xiaoyan Xu, Yun-Fei Dang, Lisen Li, Wang Shentong |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Fish Proteins
0301 basic medicine Carps animal diseases Aquatic Science Microbiology Fish Diseases 03 medical and health sciences Immune system Animals Environmental Chemistry chemistry.chemical_classification Innate immune system biology Acid phosphatase 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate Aeromonas hydrophila Grass carp 030104 developmental biology Enzyme Liver chemistry 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Alkaline phosphatase Gram-Negative Bacterial Infections Peroxidase |
| Zdroj: | Fish & Shellfish Immunology. 70:121-128 |
| ISSN: | 1050-4648 |
| Popis: | Aeromonas hydrophila is the causative agent of bacterial septicemia that is frequently observed in grass carp, Ctenopharyngodon idellus. In this study, we evaluated the biological parameters and immune enzymes in the liver of grass carp following A. hydrophila infection and quantified the alterations in liver histology using a semi-quantitative system. For the biological parameters, we found that the liver somatic index (LSI) was more sensitive than Fulton's condition factor (CF) and was significantly decreased at three days post-injection (DPI). At the immune enzyme level, the level of peroxidase (POD) in the liver significantly increased at 1 and 3 DPI. The activity of alkaline phosphatase (ALP) significantly increased at 3 DPI. Similarly, acid phosphatase (ACP) activity significantly increased at 1, 3, and 5 DPI. Histologically, the results indicated that the liver index at 3, 5, and 7 DPI was significantly higher than that of control groups. The regressive alterations as the highly variable reactions patterns and its index at 5 DPI was significantly higher than that of 1, 21 DPI, and the control groups. Based on our results, we suggest that grass carp resist A. hydrophila infection via an innate immune mechanism in the liver. The findings of this study will help elucidate the underlying mechanisms of resistance to A. hydrophila infection. |
| Databáze: | OpenAIRE |
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