Development and application of a liquid chromatography coupled to mass spectrometry method for the simultaneous determination of 23 antineoplastic drugs at trace levels
| Autor: | S. Fleury-Souverain, J. Maurin, D. Guillarme, S. Rudaz, P. Bonnabry |
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| Rok vydání: | 2022 |
| Předmět: |
Paclitaxel
Clinical Biochemistry Pharmaceutical Science Antineoplastic Agents Docetaxel Pemetrexed Irinotecan Vinblastine Analytical Chemistry Carboplatin Tandem Mass Spectrometry Drug Discovery Humans Ifosfamide Busulfan Cyclophosphamide Ganciclovir Spectroscopy Epirubicin Etoposide Methanol Daunorubicin Cytarabine Water Dacarbazine Oxaliplatin Methotrexate Doxorubicin Vincristine Fluorouracil Idarubicin Topotecan Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 221 |
| ISSN: | 1873-264X |
| Popis: | The goal of this study was to develop a method for the simultaneous quantification of 23 commonly used antineoplastic drugs in a hospital pharmacy, using ultra-high pressure liquid chromatography separation coupled to tandem mass spectrometry detection (UHPLC-MS/MS). The following drugs were investigated: 5-fluorouracil, cytarabine, ganciclovir, gemcitabine, dacarbazine, methotrexate, pemetrexed, busulfan, topotecan, rentitrexed, ifosfamide, cyclophosphamide, etoposide, irinotecan, doxorubicin/epirubicin, vincristine, docetaxel, paclitaxel, daunorubicin, idarubicin, vinblastine, oxaliplatin and carboplatin. The chromatographic separation was performed on a phenyl-hexyl column (2.1 ×100 mm, 1.7 µm) with a gradient elution of methanol and water containing 10 mM ammonium formate adjusted to pH 4.9. All compounds were analyzed in less than 13 min and detected with a triple quadrupole mass spectrometer operating in MRM mode. Limits of detection (LODs) and limits of quantification (LOQs) were comprised between 0.01 and 5 ng.mL |
| Databáze: | OpenAIRE |
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