Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis
| Autor: | Martina Bertocchi, Roberta Salaroli, Irvin Tubon, Maria Laura Bacci, Giovanni Dothel, Mercedes Fernandez, Chiara Bernardini, Monica Forni, Augusta Zannoni, Laura Calzà |
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| Přispěvatelé: | Bernardini C., Bertocchi M., Zannoni A., Salaroli R., Tubon I., Dothel G., Fernandez M., Bacci M.L., Calza L., Forni M. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Vascular Wall-MSC
Lipopolysaccharides Chemokine 040301 veterinary sciences Angiogenesis Swine Neovascularization Physiologic Stimulation Lipopolysaccharide Regenerative medicine 0403 veterinary science Transcriptome 03 medical and health sciences Vascular Wall-MSCs Animals Cells Cultured 030304 developmental biology Secretome Pig animal model 0303 health sciences lcsh:Veterinary medicine General Veterinary biology Chemistry Animal Mesenchymal stem cell Mesenchymal Stem Cells 04 agricultural and veterinary sciences General Medicine In vitro Cell biology Angiogenesi Mesenchymal Stem Cell Gene Expression Regulation biology.protein lcsh:SF600-1100 Endothelium Vascular Stem cell Research Article |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 15, Iss 1, Pp 1-12 (2019) |
| ISSN: | 1746-6148 |
| Popis: | Background MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines. Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall–Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation. Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis. Results Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10. LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation. Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs. Conclusions The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine. |
| Databáze: | OpenAIRE |
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