Fate mapping of neural stem cell niches reveals distinct origins of human cortical astrocytes
| Autor: | Denise E. Allen, Kevin C. Donohue, Cathryn R. Cadwell, David Shin, Matthew G. Keefe, Vikaas S. Sohal, Tomasz J. Nowakowski |
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| Rok vydání: | 2022 |
| Předmět: |
Multidisciplinary
General Science & Technology Neurogenesis 1.1 Normal biological development and functioning Primary Cell Culture Neurosciences Neocortex Stem Cell Research Article Neural Stem Cells Underpinning research Astrocytes Neurological Humans Cell Lineage Stem Cell Research - Nonembryonic - Non-Human Stem Cell Niche |
| Zdroj: | Science (New York, N.Y.), vol 376, iss 6600 Science |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.abm5224 |
| Popis: | Progenitors of the developing human neocortex reside in the ventricular and outer subventricular zones (VZ and OSVZ, respectively). However, whether cells derived from these niches have similar developmental fates is unknown. By performing fate mapping in primary human tissue, we demonstrate that astrocytes derived from these niches populate anatomically distinct layers. Cortical plate astrocytes emerge from VZ progenitors and proliferate locally, while putative white matter astrocytes are morphologically heterogeneous and emerge from both VZ and OSVZ progenitors. Furthermore, via single-cell sequencing of morphologically defined astrocyte subtypes using Patch-seq, we identify molecular distinctions between VZ-derived cortical plate astrocytes and OSVZ-derived white matter astrocytes that persist into adulthood. Together, our study highlights a complex role for cell lineage in the diversification of human neocortical astrocytes. |
| Databáze: | OpenAIRE |
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