Imaging lutein and zeaxanthin in the human retina with confocal resonance Raman microscopy
| Autor: | Aruna Gorusupudi, Linjia Shi, Arunkumar Ranganathan, Paul S. Bernstein, Jeanne M. Frederick, Fu-Yen Chang, Gregory T. Rognon, Binxing Li, Evan W. George |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lutein endocrine system genetic structures Confocal Outer plexiform layer Xanthophylls Retina 03 medical and health sciences chemistry.chemical_compound symbols.namesake 0302 clinical medicine Zeaxanthins Microscopy medicine Humans Carotenoid chemistry.chemical_classification Multidisciplinary Microscopy Confocal food and beverages Biological Sciences eye diseases Zeaxanthin 030104 developmental biology medicine.anatomical_structure chemistry 030221 ophthalmology & optometry symbols Biophysics sense organs Raman spectroscopy |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 |
| Popis: | Lutein and zeaxanthin are xanthophyll carotenoids that are highly concentrated in the human macula, where they protect the eye from oxidative damage and improve visual performance. Distinguishing lutein from zeaxanthin in images of the human retina in vivo or in donor eye tissues has been challenging because no available technology has been able to reliably differentiate between these two carotenoids, which differ only in the position of one C = C bond. Here, we report the differential distributions of lutein and zeaxanthin in human donor retinas mapped with confocal resonance Raman microscopy. Zeaxanthin is highly concentrated in the fovea, extending from the inner to the outer limiting membranes, with especially high concentrations in the outer plexiform layer, while lutein is much more diffuse at relatively lower concentration. Our results imply that zeaxanthin may play a more important role than lutein in human macular health and disease. |
| Databáze: | OpenAIRE |
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