The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms
| Autor: | Nikolaj Rittig, Anders Etzerodt, Sidsel Rødgaard-Hansen, Holger Jon Møller, Morten Andersen, Henning Grønbæk, Marlene Christina Nielsen, Søren K. Moestrup |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine LPS medicine.medical_treatment Immunology Antigens Differentiation Myelomonocytic Receptors Cell Surface Biology protease inhibitor 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antigens CD In vivo medicine Humans Immunology and Allergy Macrophage Lectins C-Type PKC Receptor PMA Inflammation Protease integumentary system Macrophages Zymosan Cell Biology In vitro Cell biology Mannose-Binding Lectins 030104 developmental biology chemistry 030220 oncology & carcinogenesis Tetradecanoylphorbol Acetate CD163 Biomarkers Mannose Receptor Mannose receptor |
| Zdroj: | Nielsen, M C, Andersen, M N, Rittig, N, Rødgaard-Hansen, S, Grønbæk, H, Moestrup, S K, Møller, H J & Etzerodt, A 2019, ' The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms ', Journal of Leukocyte Biology, vol. 106, no. 5, pp. 1129-1138 . https://doi.org/10.1002/JLB.3A1218-500R Nielsen, M C, Andersen, M N, Rittig, N, Rødgaard-Hansen, S, Grønbaek, H, Moestrup, S K, Møller, H J & Etzerodt, A 2019, ' The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms ', Journal of Leukocyte Biology, vol. 106, no. 5, pp. 1129-1138 . https://doi.org/10.1002/JLB.3A1218-500R |
| Popis: | The hemoglobin receptor CD163 and the mannose receptor CD206 are both expressed on the surface of human macrophages. Upon inflammatory activation, the receptors are shed from the macrophage surface generating soluble products. The plasma concentration of both soluble CD163 (sCD163) and soluble CD206 (sCD206) are increased in several diseases, including inflammatory conditions and cancer. Here, we show that in contrast to CD163, LPS-mediated shedding of CD206 in humans is slow and a result of indirect signaling. Although both sCD163 and sCD206 were increased in response to LPS stimulation in vivo, only CD163 was shed from LPS-stimulated macrophages in vitro. Although both sCD163 and sCD206 were released from cultured macrophages stimulated with zymosan and PMA, shedding of CD206 was generally slower and less efficient and not reduced by inhibitors against the major protease classes. These data indicate that CD163 and CD206 are shed from the macrophages by very different mechanisms potentially involving distinctive inflammatory processes. |
| Databáze: | OpenAIRE |
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