Molecular characterization of a deletion-prone region of plasmid pAE1 of Alcaligenes eutrophus H1
| Autor: | Wei-Yuan Chow, Shieh-Shiuh Kung, Wen-Lin Lee, Chen-Kuen Wang, Yi-Mi Wu |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping Microbiology Recombinases chemistry.chemical_compound Restriction map Plasmid Sequence Homology Nucleic Acid Recombinase Alcaligenes Amino Acid Sequence Molecular Biology Peptide sequence Gene Repetitive Sequences Nucleic Acid Sequence Deletion Recombination Genetic Genetics Base Sequence Integrases Sequence Homology Amino Acid biology Sequence Analysis DNA biology.organism_classification Molecular biology Restriction enzyme chemistry Chromosome Inversion DNA Nucleotidyltransferases DNA Transposable Elements DNA Research Article Plasmids |
| Zdroj: | Journal of Bacteriology. 177:4157-4161 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.177.14.4157-4161.1995 |
| Popis: | A 93-kb region (D region) of plasmid pAE1 of Alcaligenes eutrophus H1 has been found to have a high rate of spontaneous deletion. In this study, we constructed a restriction endonuclease map and carried out limited sequencing of an approximately 100-kb region from pAE1 which includes the D region (the deleted region) in order to detect and characterize repetitive sequences. Two types of repetitive sequences, the R1 and R2 sequences, were observed to flank the D region; within the D region are three copies of insertion element ISAE1. The R1 and R2 sequences are arranged in direct and inverted orientations, respectively. Molecular analysis of the end product of the deletion is consistent with the hypothesis that the loss of the D-region DNA is the result of recombination between two copies of the R1 sequence. The R1 sequence encodes a 415-amino-acid protein which exhibits substantial sequence similarity to the lambda integrase family of site-specific recombinases. Its genetic function remains to be determined. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|