Depolarization drives beta-Catenin into neuronal spines promoting changes in synaptic structure and function
| Autor: | Sachiko Murase, Erin M. Schuman, Eric A. Mosser |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Dendritic spine Neuroscience(all) Recombinant Fusion Proteins Green Fluorescent Proteins Nerve Tissue Proteins Pyridinium Compounds Biology Hippocampus Synaptic Transmission Membrane Potentials Potassium Chloride Actin remodeling of neurons Postsynaptic potential Animals Enzyme Inhibitors Cells Cultured beta Catenin Fluorescent Dyes Neuronal Plasticity Cadherin General Neuroscience Intracellular Signaling Peptides and Proteins Membrane Proteins Depolarization Dendrites Protein-Tyrosine Kinases Actin cytoskeleton Cadherins Synapsins Dendritic filopodia Cell biology Rats Quaternary Ammonium Compounds Cytoskeletal Proteins Luminescent Proteins Protein Transport Animals Newborn Catenin Mutation Synapses Trans-Activators Tyrosine Indicators and Reagents Disks Large Homolog 4 Protein |
| Zdroj: | Neuron |
| Popis: | Activity-induced changes in adhesion molecules may coordinate presynaptic and postsynaptic plasticity. Here, we demonstrate that beta-catenin, which mediates interactions between cadherins and the actin cytoskeleton, moves from dendritic shafts into spines upon depolarization, increasing its association with cadherins. beta-catenin's redistribution was mimicked or prevented by a tyrosine kinase or phosphatase inhibitor, respectively. Point mutations of beta-catenin's tyrosine 654 altered the shaft/spine distribution: Y654F-beta-catenin-GFP (phosphorylation-prevented) was concentrated in spines, whereas Y654E-beta-catenin-GFP (phosphorylation-mimic) accumulated in dendritic shafts. In Y654F-expressing neurons, the PSD-95 or associated synapsin-I clusters were larger than those observed in either wild-type-beta-catenin or also Y654E-expressing neurons. Y654F-expressing neurons exhibited a higher minifrequency. Thus, neural activity induces beta-catenin's redistribution into spines, where it interacts with cadherin to influence synaptic size and strength. |
| Databáze: | OpenAIRE |
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