Localisation on human chromosome 19 of three genes for cell surface antigens defined by monoclonal antibodies
| Autor: | Lloyd J. Old, W.J. Rettig, J D Brook, D.J. Shaw, H. R. Beresford |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.drug_class
Breast Neoplasms Astrocytoma Hybrid Cells Biology Monoclonal antibody Cell Line Antigen Chromosome 19 Genetics medicine Animals Humans Lymphocytes Molecular Biology Gene Genetics (clinical) Somatic Cell Hybrids Antibodies Monoclonal Chromosome Mapping Human cell Cell Surface Antigens Molecular biology Clone Cells Genes Antigens Surface Female Chromosomes Human Pair 19 |
| Zdroj: | Cytogenetic and Genome Research. 45:156-162 |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000132447 |
| Popis: | Expression of three distinct human cell surface antigens defined by monoclonal antibodies (mAbs) was examined in a series of rodent-human somatic cell hybrids retaining different subsets of human chromosomes. Cell surface reactivity with mAbs F8 and G253, detecting a 95 kilodalton (kD) glycoprotein (gp95); with mAbs F10 and A103, detecting a 50 kD glycoprotein (gp50); and with mAb S7 was found to cosegregate with human chromosome 19. However, differential antigen expression was observed with hybrids containing fragments of the 19 and hybrids constructed with different human cell types. Comparison of results from the serological typing with the presence of a number of chromosome 19 DNA markers in hybrid cells and cytogenetic analysis suggests that MSK20, the gene coding for the F10/A103 antigen gp50, is located in chromosome region 19pter→19p13.2. The genes coding for the F8/G253 antigen, gp95 (gene symbol MSK19) and the S7 antigen (MSK37) are located in region 19p13.2→19q13.2. Thus, the cell surface antigens described in this study may be used as selectable markers for specific portions of human chromosome 19. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|