Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip
| Autor: | Hojun Kim, Wonjune Kim, Seonae Jang, Honggu Chun, Gijung Kim, Min Chul Park, Daeyoung Han, Sunghoon Kim |
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| Rok vydání: | 2021 |
| Předmět: |
Serum
Materials science Scanning electron microscope Diffusion Clinical Biochemistry Nanoparticle tracking analysis Bioengineering exosomes Article Analytical Chemistry Nanopores Extracellular Vesicles Lab-On-A-Chip Devices Humans Nanotechnology nanopore General Medicine Chip Microvesicles Culture Media Nanopore diffusion-based separation Chemical engineering PC membrane Yield (chemistry) Nanoparticles Ultracentrifuge Biochemistry and Cell Biology TP248.13-248.65 Biotechnology |
| Zdroj: | Biosensors, vol 11, iss 9 Biosensors Volume 11 Issue 9 Biosensors, Vol 11, Iss 347, p 347 (2021) |
| Popis: | Extracellular vesicles (EVs) have emerged as novel biomarkers and therapeutic material. However, the small size (~200 nm) of EVs makes efficient separation challenging. Here, a physical/chemical stress-free separation of EVs based on diffusion through a nanoporous membrane chip is presented. A polycarbonate membrane with 200 nm pores, positioned between two chambers, functions as the size-selective filter. Using the chip, EVs from cell culture media and human serum were separated. The separated EVs were analyzed by nanoparticle tracking analysis (NTA), scanning electron microscopy, and immunoblotting. The experimental results proved the selective separation of EVs in cell culture media and human serum. Moreover, the diffusion-based separation showed a high yield of EVs in human serum compared to ultracentrifuge-based separation. The EV recovery rate analyzed from NTA data was 42% for cell culture media samples. We expect the developed method to be a potential tool for EV separation for diagnosis and therapy because it does not require complicated processes such as immune, chemical reaction, and external force and is scalable by increasing the nanoporous membrane size. |
| Databáze: | OpenAIRE |
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