Transforming Growth Factor Beta Stimulates Mitogenically Mouse NIH3T3 Fibroblasts and Those Cells Transformed by the EJ-H-ras Oncogene
| Autor: | Martine Pironin, Abderrahim Merzak, Yolande Dooghe, David A. Lawrence, Omar Benzakour, Philippe Vigier |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Cell type Transcription Genetic Clinical Biochemistry Genes myc Basal (phylogenetics) Mice Endocrinology Transforming Growth Factor beta Internal medicine medicine Animals Cell Line Transformed Oncogene biology Chemistry Embryo Transforming growth factor beta 3T3 Cells DNA Cell Biology Fibroblasts Transforming Growth Factor alpha Molecular biology Thymidine incorporation Kinetics Cell Transformation Neoplastic Genes ras Culture Media Conditioned biology.protein Cell Division Transforming growth factor Thymidine |
| Zdroj: | Growth Factors. 6:265-275 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977199209026933 |
| Popis: | TGF-beta 1 stimulates thymidine incorporation and the growth rate of mouse NIH3T3 fibroblasts and of those cells transformed by the EJ-H-ras oncogene (TR15 cells), in the presence and the absence of serum. Thymidine incorporation, in serum-deprived cells, is stimulated to a higher degree by 0.1-1 ng/ml of TGF-beta in NIH3T3 than in TR15 cells, which have a 10-fold higher basal level of incorporation. In both cell types TGF-beta 1 is as active, or more active than other mitogens (TGF-alpha, PDGF-AB, bFGF) at the same concentration. The growth rate of NIH3T3 cells, in low serum or serum-free (S-) medium, is stimulated by only 10 picograms/ml of TGF-beta 1, and that of TR15 cells, in S- medium, by only 1 picogram/ml. In contrast, TGF-beta 1 inhibits mitogenically unestablished mouse embryo fibroblasts and these fibroblasts immortalized spontaneously and able to grow in S- medium. It also inhibits the anchorage-independent growth of TR15 cells. NIH3T3 and TR15 cells respond, similarly, to TGF-beta activated by acification of their culture medium. The kinetics of thymidine incorporation and of activation of the c-myc proto-oncogene, observed already after 1 hr, in treated NIH3T3 and TR15 cells, suggests a direct mitogenic stimulation. The level of activated c-myc RNA is 2-fold higher at 2 hr, and subsequently decreases relatively less in the TR15 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|