| Autor: |
A.Yu. Gavrilova, I.Yu. Solokhina, I. V. Gor'kova, I. A. Gneusheva, I. N. Gagarina |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
BIO Web of Conferences, Vol 36, p 05007 (2021) |
| ISSN: |
2117-4458 |
| Popis: |
Studies have been conducted on the use of an optimized Murashige-Scoog nutrient medium to obtain healthy seeds of promising potato varieties. It was found that the use of agarized modified Murashige-Scoog nutrient medium with the addition of exometabolites of fungi of the genus Trichoderma lixii 1.5 mg/l with antimicrobial properties and the antiviral drug “Cycloferon” in a concentration of 0.01% contributes to an increase in the number of virus-free potato regenerants when the method of apical meristems and in vitro improvement is used together. The effectiveness of using the modified medium is confirmed by the following criteria: active growth of test tube potato micro-plants, which is expressed in optimal morphometric indicators on the example of the potato variety “Rosara”; the size of the isolated meristems is 10 mm, which contributes to the output of healthy plants; the survival rate of regenerating plants. It is shown that when using the modified medium, an average of 35-50% of regenerating plants were obtained, depending on the potato variety, free from M, S viruses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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