A method for studying the metabolic activity of individual tardigrades by measuring oxygen uptake using microrespirometry
| Autor: | Kai Finster, Bjarke H. Pedersen, Hans Malte, Hans Ramløv |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Physiology
Zoology Aquatic Science 01 natural sciences 03 medical and health sciences Respirometry 0103 physical sciences Respiration Energetics Tardigrada Humans 010303 astronomy & astrophysics Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology Macrobiotus macrocalix 0303 health sciences biology Reproduction Water biology.organism_classification Oxygen uptake Oxygen Metabolism Insect Science O2 respiration rate Animal Science and Zoology Tardigrade Metabolic activity Respiration rate Richtersius coronifer |
| Zdroj: | Pedersen, B H, Malte, H, Ramløv, H & Finster, K 2020, ' A method for studying the metabolic activity of individual tardigrades by measuring oxygen uptake using microrespirometry ', The Journal of Experimental Biology, vol. 223 . https://doi.org/10.1242/jeb.233072 |
| Popis: | Studies of tardigrade biology have been severely limited by the sparsity of appropriate quantitative techniques, informative on a single-organism level. Therefore, many studies rely on motility-based survival scoring and quantifying reproductive success. Measurements of O2 respiration rates, as an integrating expression of the metabolic activity of single tardigrades, would provide a more comprehensive insight into how an individual tardigrade is responding to specific environmental factors or changes in life stages. Here we present and validate a new method for determining the O2 respiration rate (nmol O2 mg−1 hour−1) of single tardigrades under steady state, using O2-microsensors. As an example, we show that the O2 respiration rate determined in MilliQ water for individuals of Richtersius coronifer and of Macrobiotus macrocalix at 22 °C was 10.8±1.8 nmol O2 mg−1 hour−1 and 13.1±2.3 nmol O2 mg−1 hour−1, respectively. |
| Databáze: | OpenAIRE |
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