Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia
| Autor: | Abu Bakar Mohd Hafifi, Abd Rahim Huda-Shakirah, Latiffah Zakaria, Nurul Nadiah Mohamad Azni, Yee Jia Kee, Masratul Hawa Mohd |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology crinum asiaticum fungi food and beverages macrophomina phaseolina Herbaceous plant hymenocallis littoralis biology.organism_classification Microbiology 030308 mycology & parasitology lcsh:QK1-989 03 medical and health sciences Infectious Diseases Crinum asiaticum lcsh:Botany Botany Ornamental plant Macrophomina phaseolina Blight Hymenocallis littoralis Internal transcribed spacer Pycnidium leaf blight 030304 developmental biology |
| Zdroj: | Mycobiology, Vol 47, Iss 4, Pp 408-414 (2019) |
| ISSN: | 2092-9323 1229-8093 |
| Popis: | Crinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015-2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on C. asiaticum and H. littoralis. Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-α) gene, the causal pathogen was identified as Macrophomina phaseolina. Phylogenetic analysis of combined dataset of ITS and TEF1-α grouped the isolates studied with other isolates of M. phaseolina from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts. |
| Databáze: | OpenAIRE |
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