The antilymphatic metastatic effect of hyaluronic acid in a mouse model of oral squamous cell carcinoma
| Autor: | Hui Chen, Min Zhang, Jing Yang, Xiaofei Tang, Min Wang, Liping Tang, Wenqi Wu, Moci Qi, Zhenchuan Tian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Mice Nude Apoptosis Cachexia Metastasis 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Tongue Hyaluronic acid Tumor Cells Cultured Medicine Animals Humans Basal cell Hyaluronic Acid Cell Proliferation Pharmacology Mice Inbred BALB C Viscosupplements business.industry medicine.disease Xenograft Model Antitumor Assays stomatognathic diseases Disease Models Animal 030104 developmental biology Lymphatic system medicine.anatomical_structure Oncology chemistry Cervical lymph nodes 030220 oncology & carcinogenesis Lymphatic Metastasis Cancer research Carcinoma Squamous Cell Molecular Medicine Female Mouth Neoplasms Lymph business Research Paper |
| Zdroj: | Cancer Biol Ther |
| Popis: | Objectives: Lymphatic metastasis is the main cause of low patient survival in cases of oral squamous cell carcinoma (OSCC). Several animal models have been established to uncover the mechanism that regulates lymph node metastasis of OSCC cells. Unfortunately, these models often take a long time to establish. The prolonged tumor burden can lead to animal cachexia, which may ultimately affect the experimental outcome. To overcome the disadvantages of these models, we established an orthotopic metastatic animal model of OSCC that showed quick lymph node metastasis potential. Results: DiR dye-labeled CAL27 cells were injected into tongue tissues of BALB/c nude mice, and the cells metastasized to lymph nodes on day 3. Metastasis was monitored using an in vivo imaging system and confirmed by histological observation. Using this model, we investigated the role of hyaluronic acid (HA) on the cervical metastasis of OSCC cells. Surprisingly, we found that the presence of HA significantly reduced the incidence of metastasis to cervical lymph nodes compared with the control group. Further analysis revealed that the presence of exogenous HA promoted mesenchymal-epithelial transition (MET) in primary tumors while reducing the metastatic potential of OSCC. Conclusion: Our findings confirmed the establishment of a fast and reliable lymphatic metastatic mouse model of OSCC that can be used for investigating metastatic mechanisms and analyzing various antimetastasis strategies. An equally important discovery is the antimetastatic property of HA, which could provide a potential therapeutic strategy for OSCC. |
| Databáze: | OpenAIRE |
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