Dentin Growth after Direct Pulp Capping with the Different Fractions of Plasma Rich in Growth Factors (PRGF) vs. MTA: Experimental Study in Animal Model

Autor:	Javier Caviedes-Bucheli, José López-López, José F Gaviño-Orduña, Sophie Román-Richon, Esther Berástegui-Jimeno, Maria Cristina Manzanares-Céspedes, Juan J. Segura-Egea, Benjamín Martín-Biedma
Rok vydání:	2021
Předmět:	0301 basic medicine Mineral trioxide aggregate Regenerative endodontics plasma rich growth factors Pulpotomy Dentistry Microscòpia de fluorescència Odontologia fluorescence microscopy Article 03 medical and health sciences 0302 clinical medicine Animal model stomatognathic system Dentin Medicine pulpotomy vital pulp therapy Fluorescence microscopy business.industry pulp capping 030206 dentistry General Medicine Pulp capping stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Pulp therapy regenerative endodontics business Gingival margin
Zdroj:	Journal of Clinical Medicine, Vol 10, Iss 3432, p 3432 (2021) Journal of Clinical Medicine Volume 10 Issue 15 Dipòsit Digital de la UB Universidad de Barcelona
ISSN:	2077-0383
DOI:	10.3390/jcm10153432
Popis:	Background: This study aimed to evaluate the area of dentin growth in rabbit incisors after pulp capping with plasma rich in growth factors (PRGF) compared with mineral trioxide aggregate (MTA) by fluorescence. Methods: twenty-seven upper and lower incisors of rabbits were divided into 4 groups: poor PRGF (F1) (n = 9 teeth), rich PRGF (F2) (n = 8 teeth), ProRoot MTA (positive control, n = 5 teeth), and untreated (NC) (negative control, n = 5). Fluorochrome markers were injected 24 h before surgery and the day before euthanasia, 28 days after the vital pulp therapy (VPT). Two transverse cuts were performed to every tooth: the first cut (A), 1 mm incisal to the gingival margin, and the second cut (B), 5 mm apical to the first cut. The sections were assessed with histomorphometric evaluation by fluorescence microscopy, comparing the dentin area between fluorescence marks and the total mineralized area. Results: The higher percentage of dentin growth was observed in the F2 group (B = 63.25%, A = 36.52%), followed by F1 (B = 57.63%, A = 30,12%) and MTA (B = 38.64%, A = 15,74%) The group with lowest percentage of dentin growth was the NC group (B = 29.22%, A = 7.82%). Significant difference (p < 0.05) was found between F2 group and MTA, also statistically significant difference has been observed comparing dentin growth areas of NC group with F1 and F2 groups. Conclusions: The application of PRGF rich and poor fraction as a pulp capping material stimulated dentin formation more intensively than MTA and NC.
Databáze:	OpenAIRE
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