Preparation of liposomes at large scale using the ethanol injection method: Effect of scale-up and injection devices
| Autor: | Hatem Fessi, Catherine Charcosset, Sébastien Urbaniak, Audrey Juban, Jean-Pierre Valour |
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| Přispěvatelé: | Laboratoire d'automatique et de génie des procédés (LAGEP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
Liposome
Aqueous solution Chromatography Chemistry ACL General Chemical Engineering Dispersity Aqueous two-phase system General Chemistry [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences Porous glass 6. Clean water Membrane Pilot plant Zeta potential [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering |
| Zdroj: | Chemical Engineering Research and Design Chemical Engineering Research and Design, 2015, 94, pp.508-515 |
| ISSN: | 0263-8762 |
| Popis: | In the present study, the preparation of liposomes using the ethanol injection technique was reported for volumes from 60 mL to 3 L. Experimental set-up with different scales and injection devices have been tested: (1) a device using a syringe for injection; (2) an experimental set-up using two pumps for injecting both organic and aqueous phases through a Shirasu Porous Glass (SPG) membrane; (3) a pilot plant using a tube dipping into the aqueous phase for injection of the organic phase; (4) a pilot plant using two pumps for injection of both organic and aqueous phases through the SPG membrane. The liposome preparations obtained were characterized for their size, polydispersity index (PDI), zeta potential, and morphology by transmission electronic microscopy (TEM). Two formulations have been tested: drug-free liposome formulation and α-tocopherol-loaded liposome formulation. For both formulations, it was shown that all experimental set-ups were appropriate for the preparation of small vesicles having narrow size distributions, with good reproducibility and stability. The pilot plant using a membrane for injection improves micromixing of the organic phase into the aqueous phase and therefore optimizes the preparation time. Evaporation of ethanol performed directly on the pilot plant has the advantages of being an easy and continuous process. |
| Databáze: | OpenAIRE |
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