[Deficiencies in C4A and C4B isotypes of human complement revealed by isoelectrofocusing and chemical reactivity of activated forms]
| Autor: | Lakhtin Vm, Kozlov Lv, D'iakov Vl, Lakhtin Mv |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
Polymorphism Genetic Chemistry Organic Chemistry Immunoblotting C4A Complement C4a Biochemistry Molecular biology Sensitivity and Specificity Complement system Complement (complexity) Blood serum Luminescent Measurements Complement C4b Humans Isoelectric Focusing Metabolism Inborn Errors |
| Zdroj: | Bioorganicheskaia khimiia. 33(4) |
| ISSN: | 0132-3423 |
| Popis: | An approach is proposed to detect deficiencies in isotypes A and B of the C4 component of human complement, based on the calculation of the ratio of their IEA activities and the ratio of their quantities determined by isoelectrofocusing of their desialated forms with chemiluminescent detection in an immunoblot. The ratios of the quantities and activities of C4A/C4B practically coincided when determined in blood serum of 20 patients, many of which had inherited deficiencies in the C4 component isotypes. |
| Databáze: | OpenAIRE |
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