Differential Patterns of Liver Proteins in Experimental Murine Hepatosplenic Schistosomiasis▿ †
| Autor: | A. La Flamme, Thomas William Jordan, Bhagyashree Manivannan, William Evan Secor, Pisana Rawson |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Immunology Blotting Western Helminthiasis Spleen Schistosomiasis Biology Microbiology Mice Immune system Blood serum Gene expression medicine Image Processing Computer-Assisted Animals Protein Isoforms Electrophoresis Gel Two-Dimensional Splenic Diseases chemistry.chemical_classification Host Response and Inflammation Principal Component Analysis Liver Diseases Proteins medicine.disease biology.organism_classification Molecular biology Schistosomiasis mansoni Disease Models Animal Infectious Diseases medicine.anatomical_structure chemistry Transferrin Mice Inbred CBA Parasitology Schistosoma mansoni |
| Popis: | Schistosoma mansonieggs produced by adult worms in the mesenteric vasculature become trapped in the liver, where they induce granulomatous lesions and strong immune responses. Infected individuals suffer from intestinal schistosomiasis (INT) in 90% of cases, whereas the remaining 10% present with severe hepatosplenic schistosomiasis (HS). The CBA/J mouse model mimics human disease, with 20% of infected mice developing hypersplenomegaly syndrome (HSS) that resembles HS and 80% developing moderate splenomegaly syndrome (MSS) similar to INT. We studied differential patterns of protein expression in livers of 20-week-infected CBA/J mice with MSS or HSS to understand the molecular changes that underlie these two disease forms. Using differential in-gel electrophoresis to identify differentially expressed protein spots, we found 80 protein spots significantly changed with infection and 35 changes specific to severe disease. In particular, the abundances of prohibitin 2, transferrin isoforms, and major urinary protein isoforms were significantly altered in HSS mice. Furthermore, annexin 5, glutathioneS-transferase pi class, andS. mansoniphosphoenolpyruvate carboxykinase expression levels changed significantly with schistosome infection. Additionally, levels of major urinary protein decreased and levels of transferrin increased significantly in the sera of HSS mice compared to levels in sera of MSS or control mice, and these differences correlated to the degree of splenomegaly. These findings indicate that the liver protein abundances differ between MSS and HSS mice and may be used for the development of diagnostic markers for the early detection of hepatosplenic schistosomiasis. |
| Databáze: | OpenAIRE |
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