Supplementary Methods, Figures 1 - 8 from Inhibition of Cholinergic Signaling Causes Apoptosis in Human Bronchioalveolar Carcinoma

Autor: Piyali Dasgupta, Brandon S. Shiflett, Yi C. Chen, Haitao Luo, A. Betts Carpenter, Cody A. Stover, Christopher A. McNees, W. Elaine Hardman, Theodore R. Witte, Aaron M. Dom, Clayton M. Crabtree, Brent A. Thornhill, Kathleen C. Brown, Jamie K. Lau
Rok vydání: 2023
Popis: PDF file - 490K, Figure S1: Cell line authentication data Figure S2: Full screen western blots for ChAT, VAChT, AChE and CHT1 Figure S3: Immunohistochemistry of ChAT and VAChT in normal human lung tissues Figure S4: Nicotine-induced ACh production in human BAC requires nAChRs, VAChT, and CHT1 Figure S5: VAChT and ChAT are expressed on human BACs Figure S6: Transfection of VAChT siRNA and Sigma-R siRNA suppress the expression of these proteins in human BACs Figure S7: Effect of VAChT siRNA on the apoptotic activity of vesamicol in nicotine-treated BACs Figure S8: Overexpression of pcDNA3-HA-Akt-CA in A549 and H358 human BAC cells
Databáze: OpenAIRE