Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches
| Autor: | Joséphine Foata, Meryl Zimmerman, Anaïs Loisier, Eve Toulza, Yann Quilichini, Jérôme Boissier, Jean-François Allienne, Olivier Rey, Jean-Pierre Pointier, Stephen Mulero |
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| Přispěvatelé: | Interactions Hôtes-Pathogènes-Environnements (IHPE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD), Sciences pour l'environnement (SPE), Centre National de la Recherche Scientifique (CNRS)-Université Pascal Paoli (UPP), Centre de recherches insulaires et observatoire de l'environnement (CRIOBE), Université de Perpignan Via Domitia (UPVD)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), This study was funded by the Région Occitanie, the European 'Fonds Européen de Développement Régional' (FEDER), the French Agency for Food, Environmental and Occupational Health & Safety (ANSES) [PNREST 2019/1/059 Molrisk], and the French 'Agence Nationale de la Recherche' (ANR) [Program HySWARM ANR-18-CE35-0001]. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Bulinus truncatus Biodiversity Corsica Environmental DNA 010603 evolutionary biology 01 natural sciences Freshwater ecosystem Freshwater snail 03 medical and health sciences lcsh:QH540-549.5 Snail communities 14. Life underwater Ecology Evolution Behavior and Systematics 030304 developmental biology Nature and Landscape Conservation Galba truncatula 0303 health sciences Ecology biology [SDE.IE]Environmental Sciences/Environmental Engineering 010604 marine biology & hydrobiology Malacology 15. Life on land biology.organism_classification Geography Threatened species Biomonitoring Metabarcoding lcsh:Ecology [SDE.BE]Environmental Sciences/Biodiversity and Ecology Potamopyrgus antipodarum |
| Zdroj: | Global Ecology And Conservation (2351-9894) (Elsevier BV), 2021-01, Vol. 25, P. e01428 (13p.) Global Ecology and Conservation, Vol 25, Iss, Pp e01428-(2021) Global Ecology and Conservation Global Ecology and Conservation, Elsevier, 2021, 25, pp.e01428. ⟨10.1016/j.gecco.2020.e01428⟩ |
| ISSN: | 2351-9894 |
| DOI: | 10.1016/j.gecco.2020.e01428⟩ |
| Popis: | International audience; To assess the effect of anthropogenic activities on ecosystems, it is of prime importance to develop new tools enabling a rapid characterization of ecological communities. Freshwater ecosystems are particularly impacted and threatened by human activities and need thorough attention to preserve their biodiversity and the ecological services they provide. Studying such ecosystems is generally difficult because the associated organisms are hard to sample and to monitor. We present a ready-to-use environmental metabarcoding protocol to characterize and monitor the freshwater gastropods communities from water samples. The efficiency of this new tool was compared to a classical malacological survey at 19 sampled sites from 10 distinct rivers distributed over Corsica Island (France). From a single water sample, our eDNA monitoring tool provided a faithful characterization of the local malacofauna compared to the results obtained from the classical malacological survey, with 97.1% of species detection confirmed by both methods. The present tool successfully detected the 11 freshwater snail species previously reported in Corsica by malacological survey but was limited at the genus level for some species. Moreover, our malacological survey allowed an update of the local distribution of a wide diversity of freshwater snails including invasive species (i.e. Potamopyrgus antipodarum and Physa acuta) as well as snail hosts of pathogens of medical and veterinary importance (i.e. Bulinus truncatus and Galba truncatula). These results strengthened a previous hypothesis of an eventual competitive interaction between B. truncatus and P. antipodarum that could limit the endemization of the uro-genital bilharziasis in Corsica |
| Databáze: | OpenAIRE |
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