Evaluation of Cell-free Protein Synthesis Using PDMS-based Microreactor Arrays
| Autor: | Yasuo Shinohara, Takahiko Nojima, Yoshinobu Baba, Teruo Fujii, Takatoki Yamamoto, Rei Kakuhata, Mami Hino |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Cell-free protein synthesis
Glycerol-3-Phosphate Dehydrogenase (NAD+) Polydimethylsiloxane Cell-Free System Chemistry Silicones Reproducibility of Results Nanotechnology Living cell Microfluidic Analytical Techniques Chip Fatty Acid-Binding Proteins Combinatorial chemistry Sensitivity and Specificity Analytical Chemistry Rats chemistry.chemical_compound Cyclophilins Template Animals Luciferase Dimethylpolysiloxanes Microreactor Luciferases Cyclophilin |
| Zdroj: | Analytical Sciencies. 24:243-246 |
| Popis: | A living cell has numerous proteins, only a few thousand of which have been identified to date. Cell-free protein synthesis is a useful and promising technique to discover and produce various proteins that might be beneficial for biotechnological, pharmaceutical, and medical applications. For this study, we evaluated the performance and the general applicability of our previously developed microreactor array chip to cell-free protein synthesis by comparisons with a commercially available system. The microreactor array chip comprises a temperature control chip made of glass and a disposable reaction chamber chip made of polydimethylsiloxane (PDMS). For evaluation of the microreactor array chip, rat adipose-type fatty acid binding protein, glyceraldehyde-3-phosphate dehydrogenase, cyclophilin, and firefly luciferase were synthesized from their respective DNA templates using a cell-free extract prepared from Escherichia coli. All these proteins were synthesized in the microreactor array chip, and their respective amounts and yields were investigated quantitatively. |
| Databáze: | OpenAIRE |
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