Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter Study
| Autor: | Maryam Saleminik, Virginia George, Kevin Judge, Anna Schmidt, Monika de Arruda Indig, Yunyao Li, Viktoriya Litvak, Angela Chen, Fred Mosqueda, Farzad Oreizy, Tatyana Delman, Yang Zeng, Sharon Graminske, Shalini Seetharaman, Nicole Kimpel, Anna Lin, Sarai Paradiso, Amanda Boerner, Michelle Dabay |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Blood Platelets
Histology Erythrocytes 030204 cardiovascular system & hematology Pathology and Forensic Medicine residual Leucocytes 03 medical and health sciences Leukocyte Count 0302 clinical medicine Enumeration Leukocytes Medicine Humans Reproducibility business.industry Platelet Count flow cytometry Limits of agreement Reproducibility of Results Cell Biology Original Articles performance evaluation Multicenter study leucodepletion Original Article Nuclear medicine business Cytometry 030215 immunology leucoreduced |
| Zdroj: | Cytometry |
| ISSN: | 1552-4930 1552-4922 |
| Popis: | The BD FACSVia™ System features novel designs in hardware, software, and instrument QC. We compared the performance of the BD FACSVia System using the BD Leucocount™ kit with the BD FACSCalibur™ flow cytometer. Leucoreduced platelet (PLT, n = 252) and red blood cell (RBC, n = 278) specimens were enrolled at four sites. Each specimen was stained in four tubes using the BD Leucocount kit reagents and acquired on the two systems. BD Leucocount Control cells (high and low) were used to evaluate the inter‐site reproducibility on the BD FACSVia System at three sites over 20 days. Deming regression and Bland–Altman analysis were performed to determine the WBC absolute counts on the BD FACSVia System vs. the BD FACSCalibur system. Assay accuracy for the range of 0–350 WBCs/µl was adequate. For samples with |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text