High mechanical property silk produced by transgenic silkworms expressing the spidroins PySp1 and ASG1
| Autor: | Xiaoxiao Wang, Jinghua Ruan, Boxiong Zhong, Shuo Zhao, Xiaoli Tang, Xiaogang Ye, Meiyu Wu |
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| Rok vydání: | 2021 |
| Předmět: |
Molecular biology
Science Transgene Silk Biochemistry Article Animals Genetically Modified Bombyx mori Argiope argentata Animals Spider silk Mechanical property Spider Multidisciplinary biology Spidroin Spiders Bombyx biology.organism_classification Cell biology SILK DNA Transposable Elements Microscopy Electron Scanning Medicine Stress Mechanical Fibroins Plasmids |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Spider silk is one of the best natural fibers with excellent mechanical properties; however, due to the visual awareness, biting behavior and territory consciousness of spiders, we cannot obtain spider silk by large-scale breeding. Silkworms have a spinning system similar to that of spiders, and the use of transgenic technology in Bombyx mori, which is an ideal reactor for producing spider silk, is routine. In this study, the piggyBac transposon technique was used to achieve specific expression of two putative spider silk genes in the posterior silk glands of silkworms: aggregate spider glue 1 (ASG1) of Trichonephila clavipes (approximately 1.2 kb) and two repetitive units of pyriform spidroin 1 (PySp1) of Argiope argentata (approximately 1.4 kb). Then, two reconstituted spider silk-producing strains, the AG and PA strains, were obtained. Finally, the toughness of the silk fiber was increased by up to 91.5% and the maximum stress was enhanced by 36.9% in PA, and the respective properties in AG were increased by 21.0% and 34.2%. In summary, these two spider genes significantly enhanced the mechanical properties of silk fiber, which can provide a basis for spidroin silk production. |
| Databáze: | OpenAIRE |
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