Early intervention with electroacupuncture prevents PTSD-like behaviors in rats through enhancing hippocampal endocannabinoid signaling
| Autor: | Han-fei Sang, Zhengwu Peng, Cui-hong Zhou, Huaning Wang, Qingrong Tan, Shan-shan Xue, Ling Liu, Quan-rui Ma, Fen Xue |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Blotting Western Hippocampus Stimulation Neuropsychological Tests Hippocampal formation Real-Time Polymerase Chain Reaction Neuroprotection Rats Sprague-Dawley Stress Disorders Post-Traumatic Small hairpin RNA 03 medical and health sciences 0302 clinical medicine Animals Medicine Maze Learning Biological Psychiatry Pharmacology Brain-derived neurotrophic factor Gene knockdown Neuronal Plasticity business.industry Endocannabinoid system Rats 030227 psychiatry Disease Models Animal Electroacupuncture business Neuroscience Endocannabinoids Signal Transduction |
| Zdroj: | Progress in Neuro-Psychopharmacology and Biological Psychiatry. 93:171-181 |
| ISSN: | 0278-5846 |
| DOI: | 10.1016/j.pnpbp.2019.03.018 |
| Popis: | Electroacupuncture (EA) is a clinically useful physiological therapy that has been recently adopted to treat several brain disorders. However, the potential role of early EA intervention in the prevention of posttraumatic stress disorder (PTSD) as well as its potential cellular and molecular mechanism has never been investigated previously. In the present study, we used an enhanced single prolonged stress (ESPS) model to access the effects of early EA intervention on the prevention of anxiety-like and fear learning behaviors, as well as the influence of the expression of post-synaptic density protein 95 (PSD95), synaptophysin (Syn), brain derived neurotrophic factor (BDNF), diacylglycerol lipase alpha (DAGLα) and cannabinoid type 1 receptor (CB1R) in the hippocampus with or without DAGLα or CB1R knockdown by a short hairpin RNA (shRNA) in the hippocampus. Moreover, the effects of electrical stimulation with different parameters on the expression of DAGLα and CB1R in the hippocampal astrocytes were also observed. The results showed that Early EA intervention improved hippocampal synaptic plasticity and ameliorated PTSD-like behaviors and also increased expression of BDNF, DAGLα and CB1R. However, either DAGLα or CB1R knockdown by a short hairpin RNA (shRNA) eliminated the neuroprotective effects of early EA intervention. Furthermore, electrical stimulation with 2/15 Hz 1 mA elevated the expression of DAGLα and CB1R. Altogether, our findings provide new insights regarding the possibility of using early EA intervention in the prevention of PTSD, and the protective effects of EA is involving the activation of DAGLα and CB1R. |
| Databáze: | OpenAIRE |
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