Flavonoids fromInula britannicareduces oxidative stress through inhibiting expression and phosphorylation of p47phoxin VSMCs

Autor: Jing Zhang, Jin-kun Wen, Hong-Bing Zhang, Yan-Yan Wang, Mei Han, Guo-yan Ma, Bin Zheng, Sui-Bing Miao
Rok vydání: 2011
Předmět:
Male
Vascular smooth muscle
Pharmaceutical Science
Flowers
In Vitro Techniques
medicine.disease_cause
Muscle
Smooth
Vascular
Rats
Sprague-Dawley
Superoxide dismutase
chemistry.chemical_compound
Western blot
Superoxides
Drug Discovery
medicine
Animals
Phosphorylation
Cells
Cultured
Flavonoids
Inflammation
Pharmacology
chemistry.chemical_classification
Reactive oxygen species
Inula
biology
medicine.diagnostic_test
Superoxide Dismutase
Tumor Necrosis Factor-alpha
Superoxide
NADPH Oxidases
Hydrogen Peroxide
General Medicine
biology.organism_classification
Molecular biology
Rats
Oxidative Stress
Gene Expression Regulation
Complementary and alternative medicine
Biochemistry
Inula britannica
chemistry
biology.protein
Molecular Medicine
Plant Preparations
Reactive Oxygen Species
Oxidative stress
Phytotherapy
Zdroj: Pharmaceutical Biology. 49:815-820
ISSN: 1744-5116
1388-0209
DOI: 10.3109/13880209.2010.550055
Popis: Inula britanica Linn. (Compositae) is a traditional Chinese medicinal herb that has been used to treat bronchitis and inflammation. The total flavonoid extracts (TFEs) isolated from its flowers can inhibit neointimal formation induced by balloon injury in vivo.To investigate the mechanism by which TFE suppresses oxidative stress generation and the subsequent inflammation response in vitro.The cultured vascular smooth muscle cells (VSMCs) form rats were exposed to oxidative stress following pretreatment with or without TFE at different concentration. Then, fluorescence staining was used to detect superoxide anion (O₂(˙-)) production, and the lever of maleic dialdehyde (MDA) and superoxide dismutase (SOD) was measured at the same time. Furthermore, tumor necrosis factor-α (TNF-α) was measured by enzyme linked immunosorbent assay (ELISA), reverse transcription-PCR and western blot were performed to detect the expression activity of p47(phox) gene, and immunoprecipitation was used to test the level of p47(phox) phosphorylation.TFE inhibited the production of O₂(˙-) induced by H₂O₂ in VSMCs, with decrease in secretion of TNF-α; elevated the activity of SOD in the medium, similar to the effect of quercetin; reduced the level of MDA in culture medium of VSMCs. The pretreatment with TFE resulted in decrease the level of p47(phox) mRNA and protein, and even p47(phox) phosphorylation in VSMCs, compared with H₂O₂ control.These findings demonstrate that TFE is capable of attenuating the oxidative stress generation and the subsequent inflammation response via preventing the overexpression and activation of p47(phox) and the increased TNF-α secretion in VSMCs in vitro.
Databáze: OpenAIRE
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