Evaluation of Anti-Prion Activity of Congo Red and its Derivatives in Experimentally Infected Hamsters
| Autor: | Stefania Villa, Paolo Mantegazza, Claudia Pollera, Maria Laura Giannino, Selina Iussich, Piera Anna Martino, Giorgio Poli, Silvio R. Bareggi, Paola Dall'Ara, Wilma Ponti, G. Carcassola, Vito M. Tranquillo |
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| Rok vydání: | 2011 |
| Předmět: |
Injections
Subcutaneous animal diseases Bovine spongiform encephalopathy Hamster Scrapie Biology Injections Prion Diseases chemistry.chemical_compound Cricetinae Drug Discovery medicine Animals Settore VET/05 - Malattie Infettive degli Animali Domestici Coloring Agents Mesocricetus Brain Congo Red New variant medicine.disease Immunohistochemistry Survival Analysis Settore CHIM/08 - Chimica Farmaceutica Virology Benzidine In vitro nervous system diseases Subcutaneous route Congo red chemistry Settore BIO/14 - Farmacologia Female |
| Zdroj: | Università degli Studi di Milano-IRIS Scopus-Elsevier |
| ISSN: | 1616-7066 0004-4172 |
| Popis: | Among transmissible spongiform encephalopathies (TSE), particularly dreadful are the bovine spongiform encephalopathy (BSE), because of its epidemic character, and the new variant of Creutzfeldt-Jakob disease (vCJD) in man, possibly related to BSE prion, through the intake of infected food. To treat TSE, many potentially therapeutic agents have been tested: some of them, among which is Congo Red (CAS 573-58-0, CR), delayed the onset of symptoms in scrapie-in-fected rodents, and some CR derivatives proved to be effective in vitro. The capacity of a synthesized CR derivative (CR-A) and of the aromatic central benzidine rings of CR (CR-B) to abrogate scrapie-induced disease in experimentally infected hamsters was assayed. CR, used as reference substance, administered i.c. after pre-incubation with the scrapie inoculum, was strongly effective in slowing the progression of the infection, while both CR-A and CR-B, administered alone or together, were not effective. Both CR-A and CR, when administered by subcutaneous route in i.c. scrapie-infected animals, prolonged the survival time in comparison to controls; CR-B was not effective. Moreover, both CR and CR-A were very effective in prolonging the survival time of i.p. scrapie-infected hamsters. The hypothesis of possible different mechanisms of interaction between CR or CR-A and the scrapie agent related to the chemical structures of the molecules is discussed. |
| Databáze: | OpenAIRE |
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