Circ_0000144 facilitates the progression of thyroid cancer via the miR-217/AKT3 pathway
| Autor: | Yu-Lin Hu, Xiao-Li Jin, Yi-Xiang Fan, Huan-Yang Shi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Small interfering RNA Apoptosis 03 medical and health sciences 0302 clinical medicine Drug Discovery Genetics Biomarkers Tumor Tumor Cells Cultured Cytotoxic T cell Humans Thyroid Neoplasms Molecular Biology Genetics (clinical) Cell Proliferation Gene knockdown Cell growth Chemistry RNA Circular Blot Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Tumor progression Cell culture 030220 oncology & carcinogenesis Cancer cell Cancer research Molecular Medicine Proto-Oncogene Proteins c-akt |
| Zdroj: | The journal of gene medicineREFERENCES. 22(12) |
| ISSN: | 1521-2254 |
| Popis: | BACKGROUND Thyroid carcinoma (TC) is the most common malignancy of the endocrine system. Circular RNA (circRNA) is vital in the regulation of tumor progression. Circ_0000144 serves as a novel oncogenic circRNA, and miR-217 is reported to inhibit the malignant phenotypes of cancer cells by targeting AKT3 in TC. The present study aimed to explore the regulatory mechanism of circ_0000144 and miR-217 in the progression of TC. METHODS Circ_0000144 expression in 32 pairs of TC tissues and different TC cell lines (including BCPAP, K1, H7H83, and TPC-1) was detected by employing a quantitative real-time polymerase chain reaction (qRT-PCR). Circ_0000144 small interfering RNA was used to establish loss-of-function models. Cell counting kit-8 (CCK-8), BrdU (5-bromo-2'-deoxyuridine) and transwell assays were utilized to verify the effects of circ_0000144 on TC cell proliferation, migration and invasion, respectively. Bioinformatics, western blotting, a luciferase reporter experiment and qRT-PCR were employed to confirm the relationships among circ_0000144, miR-217 and AKT3. RESULTS Circ_0000144 expression was remarkably elevated in TC tissues (p |
| Databáze: | OpenAIRE |
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