Rectal pre-treatment with ozonized oxygen (O3) aggravates clinic status in septic rats treated with amoxicillin/clavulanate
| Autor: | Fernando Artiles Campelo, Miguel A. García-Bello, Pedro Manuel Herráez Thomas, José Alberto Montoya-Alonso, Antonio Espinosa de los Monteros y Zayas, Bernardino Clavo Varas, José L Martín-Barrasa, Mª Candelaria Juste de Santa Ana, M. Teresa Tejedor-Junco, Charlín Méndez Cordovez, Ana Bordes Benitez |
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| Rok vydání: | 2015 |
| Předmět: |
musculoskeletal diseases
Microbiology (medical) medicine.medical_specialty medicine.drug_class Premedication medicine.medical_treatment Antibiotics Peritonitis Bacteremia Amoxicillin-Potassium Clavulanate Combination Gastroenterology Rats Sprague-Dawley Sepsis Random Allocation Ozone Internal medicine Pneumonia Bacterial medicine Animals Pleurisy Saline Escherichia coli Infections business.industry Peritoneal fluid Drug Resistance Microbial Amoxicillin medicine.disease Rats Surgery Oxygen Myocarditis Pneumonia Female business Serositis medicine.drug |
| Zdroj: | Enfermedades Infecciosas y Microbiología Clínica. 33:469-475 |
| ISSN: | 0213-005X |
| Popis: | Introduction Despite the advanced antibiotic therapies, sepsis continues being a clinical entity with high morbidity and mortality. The ozone/oxygen mixture (O3/O2) has been reported to exhibit positive effects on immunity. The aim of our study was to analyze whether (O3/O2) combined with amoxicillin/clavulanate has any influence on the morbidity and mortality of septic rats. Methods We used 48 Sprague-Dawley rats randomly allocated to 6 groups (n = 8): healthy (C), septic (I), healthy + ozone therapy (O3), septic + amoxicillin/clavulanate (AMC), septic + amoxicillin/clavulanate + ozone therapy (AMC/O3) and septic + ozone therapy (I/O3). O3/O2 was administered rectally at increasing O3 concentrations during 10 days prior to the onset of sepsis model (intraperitoneally injection of fecal material) or saline administration in healthy control rats. Later (post-inoculation), 3 days per week, O3 was also administered. Vital signs were recorded, and microbiological, hematological and histopathological studies were performed. Results The number of surviving animal/total was higher in AMC (8/8) than in AMC/O3 (4/8) p = 0.077. The percentage of surviving animals with pneumonia was higher in AMC/O3 than in AMC (100% vs 37.5%). In dead animals, AMC/O3 rats had a significantly higher percentage of lesions: Cardiac lesions, pulmonary hemorrhages and pleuritis (100%) and serositis/peritonitis (75%). Only Escherichia coli (2 different biotypes) was isolated from blood and/or peritoneal fluid from all infected groups. A significant decrease in the percentage of band neutrophils from the surviviors belonging to AMC/O3 vs AMC was observed (p Conclusion Rectal pre-treatment with O3/O2 aggravates clinic status in septic rats treated with amoxicillin/clavulanate. |
| Databáze: | OpenAIRE |
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