Microarray analysis identifies Autotaxin, a tumour cell motility and angiogenic factor with lysophospholipase D activity, as a specific target of cell transformation by v-Jun
| Autor: | Elizabeth J. Black, Timothy Clair, David A. F. Gillespie, Paul E. Neiman, Jeffrey J. Delrow |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Oncogene Protein p65(gag-jun) Down-Regulation Chick Embryo Biology medicine.disease_cause Paracrine signalling Multienzyme Complexes Genetics medicine Animals Pyrophosphatases Autocrine signalling Molecular Biology Transcription factor Gene Glycoproteins Oligonucleotide Array Sequence Analysis Phosphoric Diester Hydrolases Microarray analysis techniques Glucose-6-Phosphate Isomerase Molecular biology Up-Regulation Cell Transformation Neoplastic Lysophospholipase Phosphodiesterase I Autotaxin Carcinogenesis |
| Zdroj: | Oncogene. 23:2357-2366 |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1207377 |
| Popis: | We have used chicken cDNA microarrays to investigate gene-expression changes induced during transformation of chick embryo fibroblasts (CEF) by the viral Jun oncoprotein encoded by ASV17. This analysis reveals that v-Jun induces increases and decreases of varying magnitude in the expression of genes involved in diverse cellular functions, most of which have not been detected in previous screens for putative v-Jun targets. In all, 27 individual genes were identified, whose expression is increased threefold or more in v-Jun-transformed cells, including genes involved in energy generation, protein synthesis, and gene transcription. Interestingly, this group includes the hypoxia-inducible factor-1 alpha (Hif-1alpha) transcription factor and the glycolytic enzyme enolase, suggesting that adaptation to hypoxia could play a role in tumorigenesis by v-Jun. We also identified 32 genes whose expression is decreased threefold or more, including chaperones, components of the cytoskeleton, and, unexpectedly, DNA replication factors. The gene whose expression is upregulated most dramatically (approximately 100-fold) encodes Autotaxin (ATX), a secreted tumor motility-promoting factor with lysophospholipase D activity. Strikingly, v-Jun-transformed CEF secrete catalytically active ATX and chemotactic activity, which can be detected in conditioned medium. ATX is not detectably expressed in normal CEF or CEF transformed by the v-Src or v-Myc oncoproteins, indicating that induction of this putative autocrine/paracrine factor is a specific consequence of cell transformation by v-Jun. ATX has been implicated in both angiogenesis and invasion, and could therefore play an important role in tumorigenesis by v-Jun in vivo. |
| Databáze: | OpenAIRE |
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