Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes
| Autor: | Kalle Kirsimäe, Nataša Škalko-Basnet, Sveinung Gaarden Ingebrigtsen, Tavo Romann, Karin Kogermann, Vambola Kisand, Ivo Laidmäe, Irja Alainezhad Kjærvik, Urmas Joost, Andres Meos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Liposome
chloramphenicol Polyvinylpyrrolidone Chemistry VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Pharmacology: 728 Pharmaceutical Science film hydration Electrospinning Article Solvent RS1-441 Pharmacy and materia medica Chemical engineering Nanofiber Amphiphile liposome amphiphilic nanofibers medicine Extrusion VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728 Particle size electrospinning drug release medicine.drug |
| Zdroj: | Pharmaceutics, Vol 13, Iss 1742, p 1742 (2021) Pharmaceutics Volume 13 Issue 11 |
| Popis: | The hydration of phospholipids, electrospun into polymeric nanofibers and used as templates for liposome formation, offers pharmaceutical advantages as it avoids the storage of liposomes as aqueous dispersions. The objective of the present study was to electrospin and characterize amphiphilic nanofibers as templates for the preparation of antibiotic-loaded liposomes and compare this method with the conventional film-hydration method followed by extrusion. The comparison was based on particle size, encapsulation efficiency and drug-release behavior. Chloramphenicol (CAM) was used at different concentrations as a model antibacterial drug. Phosphatidylcoline (PC) with polyvinylpyrrolidone (PVP), using ethanol as a solvent, was found to be successful in fabricating the amphiphilic composite drug-loaded nanofibers as well as liposomes with both methods. The characterization of the nanofiber templates revealed that fiber diameter did not affect the liposome size. According to the optical microscopy results, the immediate hydration of phospholipids deposited on the amphiphilic nanofibers occurred within a few seconds, resulting in the formation of liposomes in water dispersions. The liposomes appeared to aggregate more readily in the concentrated than in the diluted solutions. The drug encapsulation efficiency for the fiber-hydrated liposomes varied between 14.9 and 28.1% and, for film-hydrated liposomes, between 22.0 and 77.1%, depending on the CAM concentrations and additional extrusion steps. The nanofiber hydration method was faster, as less steps were required for the in-situ liposome preparation than in the film-hydration method. The liposomes obtained using nanofiber hydration were smaller and more homogeneous than the conventional liposomes, but less drug was encapsulated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|