Upregulation of bromodomain PHD finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival
| Autor: | Juan Miao, Xiaohao Huang, Suping Han, Ranran Tang, Min Zhang, Lei Xu, Huan Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Protein subunit
Apoptosis Nerve Tissue Proteins Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Biomarkers Tumor Tumor Cells Cultured medicine Humans Neoplasm Invasiveness Molecular Biology Transcription factor Cell Proliferation 030304 developmental biology Ovarian Neoplasms 0303 health sciences Cancer cell proliferation Antigens Nuclear Cell Biology Middle Aged Prognosis medicine.disease Bromodomain Gene Expression Regulation Neoplastic Survival Rate PHD finger 030220 oncology & carcinogenesis Cancer research Female Ovarian cancer Transcription Factors |
| Zdroj: | Biochemistry and Cell Biology. 99:304-312 |
| ISSN: | 1208-6002 0829-8211 |
| Popis: | Bromodomain PHD finger transcription factor (BPTF) is a core subunit of the nucleosome-remodeling factor (NURF) complex, which plays an important role in the development of several cancers. However, it is unknown whether BPTF regulates the progression of ovarian cancer (OC). To investigate this, we measured the relative expression levels of BPTF in OC cell lines and tissues using Western blot and immunohistochemistry, respectively, and the results were analyzed using the χ2 test. We also examined the effects from BPTF knockdown on the proliferation, migration, invasiveness, and apoptosis of OC cell lines. Mechanistic studies revealed that these effects were achieved through simultaneous modulation of multiple signaling pathways. We found that BPTF was highly expressed in OC cell lines and tissues compared with a normal human ovarian epithelial cell line and non-cancerous tissues (P < 0.05). These results are also supported by the public RNA-seq data. BPTF overexpression was correlated with a poor prognosis for OC patient survival (P < 0.05). In vitro experiments revealed that the downregulation of BPTF inhibited OC cell proliferation, colony formation, migration, and invasiveness, and induced apoptosis. BPTF knockdown also affected the epithelial–mesenchymal transition (EMT) signaling pathways and induced the cleavage of apoptosis-related proteins. Consequently, BPTF plays a critical role in OC cell survival, and functions as a potential therapeutic target for OC. |
| Databáze: | OpenAIRE |
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