Vaginal Cytology of the Laboratory Rat and Mouse
| Autor: | Linda Kooistra, Greg Travlos, Michelle C. Cora |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
endocrine system
medicine.medical_specialty Physiology Estrous Cycle Biology Toxicology Pathology and Forensic Medicine Mice Cytology Cell density medicine Animals Coloring Agents Vaginal cytology Molecular Biology reproductive and urinary physiology Vaginal Smears Gynecology Estrous cycle urogenital system Cell Biology Rats Laboratory rat Wet mount Vagina Female Functional status |
| Zdroj: | Toxicologic Pathology. 43:776-793 |
| ISSN: | 1533-1601 0192-6233 |
| DOI: | 10.1177/0192623315570339 |
| Popis: | Microscopic evaluation of the types of cells present in vaginal smears has long been used to document the stages of the estrous cycle in laboratory rats and mice and as an index of the functional status of the hypothalamic–pituitary–ovarian axis. The estrous cycle is generally divided into the four stages of proestrus, estrus, metestrus, and diestrus. On cytological evaluation, these stages are defined by the absence, presence, or proportion of 4 basic cell types as well as by the cell density and arrangement of the cells on the slide. Multiple references regarding the cytology of the rat and mouse estrous cycle are available. Many contemporary references and studies, however, have relatively abbreviated definitions of the stages, are in reference to direct wet mount preparations, or lack comprehensive illustrations. This has led to ambiguity and, in some cases, a loss of appreciation for the encountered nuances of dividing a steadily moving cycle into 4 stages. The aim of this review is to provide a detailed description, discussion, and illustration of vaginal cytology of the rat and mouse estrous cycle as it appears on smears stained with metachromatic stains. |
| Databáze: | OpenAIRE |
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