Purification of Pneumolysin
| Autor: | Seymour J. Klebanoff, Clare N. Shumway |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Erythrocytes
Lysis Immunology Biology Hemolysin Proteins Microbiology Chromatography DEAE-Cellulose Crenation Bacterial Proteins medicine Animals Humans Trypsin Polyacrylamide gel electrophoresis Mercaptoethanol Pneumolysin Erythrocyte fragility Osmotic Fragility Cholesterol Streptococcus pneumoniae Infectious Diseases Biochemistry Ammonium Sulfate Spectrophotometry Injections Intravenous Hydroxymercuribenzoates Pathogenic Mechanisms Ecology and Epidemiology Electrophoresis Polyacrylamide Gel Parasitology Specific activity Rabbits medicine.drug |
| Zdroj: | Infection and Immunity. 4:388-392 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.4.4.388-392.1971 |
| Popis: | A procedure for the purification of pneumolysin has been developed. Its use results in a 96-fold increase in specific activity from the original crude pneumococcal extract to the final purified product. The purified material is heat-labile and is inactivated by trypsin and cholesterol; inactivation by p -hydroxymercuribenzoate can be reversed by mercaptoethanol. Intravenous administration of the material to rabbits results in crenation, increased osmotic fragility, and intravascular lysis of the erythrocytes of the animal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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