Evaluation of antioxidant enzymes performances and DNA damage induced by bisphenol A and diisobutylphthalate in Hyperiodrilus africanus-earthworms
| Autor: | Solomon Oladapo Rotimi, Rhoda Ayoola, Oluwatosin Dosumu, Olanrewaju Olujimi, A. A. Aladesida, Oluwafunmilayo O. Olayinka |
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| Rok vydání: | 2020 |
| Předmět: |
endocrine system
Bisphenol A Antioxidant DNA damage Health Toxicology and Mutagenesis medicine.medical_treatment 0208 environmental biotechnology 02 engineering and technology 010501 environmental sciences Toxicology 01 natural sciences Antioxidants Superoxide dismutase chemistry.chemical_compound lcsh:Environmental pollution medicine Earthworms Food science 0105 earth and related environmental sciences chemistry.chemical_classification biology urogenital system Earthworm Public Health Environmental and Occupational Health biology.organism_classification BPA DIBP Enzymes 020801 environmental engineering Enzyme Point of delivery chemistry lcsh:TD172-193.5 biology.protein hormones hormone substitutes and hormone antagonists Peroxidase |
| Zdroj: | Emerging Contaminants, Vol 6, Iss, Pp 1-9 (2020) |
| ISSN: | 2405-6650 |
| DOI: | 10.1016/j.emcon.2019.10.001 |
| Popis: | Background: Bisphenol A (BPA) and diisobuthylphthalate (DIBP) have found wide applications as plasticizers; however, they are covalently bonded to materials and are subsequently leached into the environment. Methods: This study evaluated the effects of BPA, DIBP and mixtures of BPA and DIBP on the antioxidant enzymes [superoxide dismutase (SOD), peroxidase (POD)] activities using standard methods and DNA profile of earthworm (Hyperiodrilus africanus) using randomly amplified polymerase chain reaction (RAPD-PCR) method. A total of 2 kg soil samples collected from FADAMA Farm, Federal University of Agriculture, Abeokuta were spiked to obtain 1, 2.5, 5 and 10 ppm of BPA, DIBP and mixtures of BPA and DIBP. Earthworms were exposed to the unpolluted and spiked soil samples and were collected on the 7th and 14th day for SOD, POD and DNA analysis. Results: SOD activity was more pronounced at 5 and 10 ppm concentrations. POD activity was increased in all concentrations, including the control, meanwhile POD activity induced by BPA and DIBP was significantly higher (p |
| Databáze: | OpenAIRE |
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