Rat white adipocytes activate p85/p110 PI3K and induce PM GLUT4 in response to adrenoceptor agonists or aluminum fluoride
| Autor: | Y Ohsaka, Y Nomura |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Agonist Male medicine.medical_specialty Adrenergic receptor medicine.drug_class Adipocytes White Adrenergic Dioxoles Pertussis toxin 03 medical and health sciences chemistry.chemical_compound Fluorides Norepinephrine Physiology (medical) Adipocyte Internal medicine Dobutamine Receptors Adrenergic beta medicine Animals Phosphatidylinositol Rats Wistar Aluminum Compounds Glucose Transporter Type 4 biology Cell Membrane Glucose transporter General Medicine Adrenergic beta-Agonists Rats Class Ia Phosphatidylinositol 3-Kinase Enzyme Activation 030104 developmental biology Endocrinology chemistry biology.protein GLUT4 |
| Zdroj: | Physiology international. 103(1) |
| ISSN: | 2498-602X |
| Popis: | Adipocyte responses to adrenergic and s-adrenoceptor(-AR) (adrenoceptor) regulation are not sufficiently understood, and information helpful for elucidating the adrenoceptor-responsive machinery is insufficient. Here we show by using immunoprecipitated kinase analysis with a phosphatidylinositol 3-kinase (PI3K) p85 antibody that PI3K activation was induced by treatment with 10 or 100 µM norepinephrine (NE) for 15 min or with 10 mM aluminum fluoride (AF, a guanosine triphosphate (GTP)-binding (G) protein activator) for 20 min in white adipocytes (rat epididymal adipocytes) and that treatment with pertussis toxin (PTX, a G-protein inactivator) inhibited PI3K activation induced by the 20-min treatment with AF in the cells. In addition, western blot analysis revealed that glucose transporter 4 (GLUT4) level in the adipocyte plasma membrane (PM) fraction was increased by treatment with 10 µM NE, 100 µM dobutamine (DOB, a s1-AR agonist), or 0.1 µM CL316243 (CL, a s3-AR agonist) for 30 min or with 10 mM AF for 20 min. NE or AF treatment triggered 2-deoxyglucose (2-DG) uptake into adipocytes under the above conditions. Our results advance the understanding of responses to adrenoceptor regulation in white adipocytes and provide possible clues for clarifying the machinery involved in adrenergic and s-AR responses in the cells. |
| Databáze: | OpenAIRE |
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