Preparation and microbial decomposition of synthetic [14C]ligins
| Autor: | William J. Connors, R D Bleam, W. F. Hackett, J. G. Zeikus, T. K. Kirk |
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| Jazyk: | angličtina |
| Rok vydání: | 1975 |
| Předmět: |
Magnetic Resonance Spectroscopy
Time Factors Spectrophotometry Infrared Alcohol macromolecular substances complex mixtures Lignin Polyporaceae chemistry.chemical_compound Species Specificity Organic chemistry Carbon Radioisotopes chemistry.chemical_classification Multidisciplinary biology fungi technology industry and agriculture Fungi food and beverages Polymer Biodegradation Decomposition Kinetics chemistry Polymerization biology.protein Spectrophotometry Ultraviolet Agaricales Peroxidase Coniferyl alcohol Research Article |
| Popis: | A definitive assay for microbiological and biochemical research on the biodegradation of lignin was developed using radioactive synthetic lignins specifically labeled in the side chains, aromatic rings or in the methoxyl groups. The [14C]lignins were prepared by oxidative polymerization with peroxidase and H2O2 Of specifically labeled coniferyl alcohol (4-hydroxy-3-methyoxycinnamyl alcohol). The synthetic polymers were shown by spectroscopic and chemical methods to contain the same intermonomer linkages found in natural lignins. Incubation of the [14C]lignins with known lignin-degrading fungi and with a forest soil resulted in 14CO2 evolution. |
| Databáze: | OpenAIRE |
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