Prediction of a miRNA‐mRNA functional synergistic network for cervical squamous cell carcinoma

Autor: Yanjie Deng, Xiaohui Yu, Dan Sun, Huali Wang, Jiyong Jiang, Lu Han, Rui Cao
Rok vydání: 2019
Předmět:
0301 basic medicine
Gene Expression
Uterine Cervical Neoplasms
cervical intraepithelial neoplasia
Biology
Cervical intraepithelial neoplasia
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Prostate cancer
0302 clinical medicine
Cyclin D1
Gene interaction
microRNA
Biomarkers
Tumor
medicine
Humans
Gene Regulatory Networks
Protein Interaction Maps
RNA
Messenger
lcsh:QH301-705.5
Research Articles
miRNA
Cervical cancer
Cyclin-dependent kinase 1
Gene Expression Profiling
Computational Biology
Uterine Cervical Dysplasia
GEO
medicine.disease
differentially expressed gene
Gene Expression Regulation
Neoplastic
MicroRNAs
030104 developmental biology
lcsh:Biology (General)
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
cervical squamous cell carcinoma
Cancer research
Female
Aurora Kinase A
Transcriptome
Research Article
Signal Transduction
Zdroj: FEBS Open Bio, Vol 9, Iss 12, Pp 2080-2092 (2019)
FEBS Open Bio
ISSN: 2211-5463
Popis: Cervical squamous cell carcinoma (CSCC) accounts for a significant proportion of cervical cancer; thus, there is a need for novel and noninvasive diagnostic biomarkers for this malignancy. In this study, we performed integrated analysis of a dataset from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) and differentially expressed miRNAs (DEmiRNAs) between CSCC, cervical intraepithelial neoplasia (CIN) and healthy control subjects. We further established protein‐protein interaction and DEmiRNA‐target gene interaction networks, and performed functional annotation of the target genes of DEmiRNAs. In total, we identified 1375 DEGs and 19 DEmiRNAs in CIN versus normal control, and 2235 DEGs and 33 DEmiRNAs in CSCC versus CIN by integrated analysis. Our protein‐protein interaction network indicates that the common DEGs, Cyclin B/cyclin‐dependent kinase 1 (CDK1), CCND1, ESR1 and Aurora kinase A (AURKA), are the top four hub genes. P53 and prostate cancer were identified as significantly enriched signaling pathways of common DEGs and DEmiRNA targets, respectively. We validated that expression levels of three DEGs (TYMS, SASH1 and CDK1) and one DEmiRNA of hsa‐miR‐99a were altered in blood samples of patients with CSCC. In conclusion, a total of four DEGs (TYMS, SASH1, CDK1 and AURKA) and two DEmiRNAs (hsa‐miR‐21 and hsa‐miR‐99a) may be involved in the pathogenesis of CIN and the progression of CIN into CSCC. Of these, TYMS is predicted to be regulated by hsa‐miR‐99a and SASH1 to be regulated by hsa‐miR‐21.
Here, we performed integrated analysis of a Gene Expression Omnibus database dataset to identify differentially expressed mRNAs and differentially expressed miRNAs in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma. We identified a total of four differentially expressed genes (TYMS, SASH1, Cyclin B/cyclin‐dependent kinase 1 and Aurora kinase A) and two differentially expressed miRNAs (hsa‐miR‐21 and hsa‐miR‐99a) that may be involved in the pathogenesis of CIN and the progression of CIN into cervical squamous cell carcinoma.
Databáze: OpenAIRE
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