A Chemically Synthesized Capture Agent Enables the Selective, Sensitive, and Robust Electrochemical Detection of Anthrax Protective Antigen
| Autor: | Heather D. Agnew, Blake Farrow, Sung Yang, Amethist S. Finch, Sung A Hong, Matthew B. Coppock, Errika C. Romero, Bert T. Lai, Kaycie Deyle, Dimitra N. Stratis-Cullum, James R. Heath |
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| Rok vydání: | 2013 |
| Předmět: |
Materials science
Bacterial Toxins General Physics and Astronomy Enzyme-Linked Immunosorbent Assay Peptide medicine.disease_cause Blood serum Limit of Detection medicine General Materials Science Peptide library Detection limit chemistry.chemical_classification Antigens Bacterial Chromatography medicine.diagnostic_test biology General Engineering Electrochemical Techniques biology.organism_classification Molecular biology Bacillus anthracis chemistry Immunoassay Biosensor Exotoxin |
| Zdroj: | ACS Nano. 7:9452-9460 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/nn404296k |
| Popis: | We report on a robust and sensitive approach for detecting protective antigen (PA) exotoxin from Bacillus anthracis in complex media. A peptide-based capture agent against PA was developed by improving a bacteria display-developed peptide into a highly selective biligand through in situ click screening against a large, chemically synthesized peptide library. This biligand was coupled with an electrochemical enzyme-linked immunosorbent assay utilizing nanostructured gold electrodes. The resultant assay yielded a limit of detection of PA of 170 pg/mL. (2.1 pM) in buffer, with minimal sensitivity reduction in 1% serum. The powdered capture agent could be stably stored for several days at 65 °C, and the full electrochemical biosensor showed no loss of performance after extended storage at 40 °C. The engineered stability and specificity of this assay should be extendable to other cases in which biomolecular detection in demanding environments is required. |
| Databáze: | OpenAIRE |
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