The relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia

Autor:	Li Wang, Hui Cen, Dongrui Mao, Dongcai Wu, Yuqiao Mo, Xue Wang, Linmei Zheng, Xiaoju Chen
Rok vydání:	2020
Předmět:	Messenger RNA Eclampsia business.industry Obstetrics and Gynecology Human leukocyte antigen medicine.disease Positive correlation female genital diseases and pregnancy complications Protein expression Blot Andrology HLA-G medicine Diagnostic biomarker business reproductive and urinary physiology
Zdroj:	Ginekologia polska.
ISSN:	2543-6767
Popis:	Objectives: To investigate the relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia. Material and methods: Real-time PCR (qRT-PCR) was used to detect the hsa_circ_0051326 expression level. Enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and western blotting were used to detect HLA-G expression in blood in 50 patients with pre-eclampsia and 50 normal pregnant women. Results: HLA-G protein expression level was decreased significantly in the blood of patients with pre-eclampsia. hsa_circ_0051326 and HLA-G mRNA in blood were decreased significantly in the pre-eclampsia patients compared with normal pregnant women. There was a positive correlation between the expression of serum hsa_circ_0051326 with HLA-G mRNA. Conclusions: Serum hsa_circ_0051326 was a new diagnostic biomarker for pre-eclampsia with equivalent efficiency of HLA-G. Maternal serum hsa_circ_0051326 level may pre-diagnose potentially pre-eclampsia before delivery.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02fe004e7da4d2db2f383e1437193468 https://pubmed.ncbi.nlm.nih.gov/35072244 Zobrazit plný text záznamu