Identification of aTorpedohomolog of Sam68 that interacts with the synapse organizing protein rapsyn
| Autor: | Paul F. Worley, Eric T. Fung, Anthony Lanahan, Richard L. Huganir |
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| Rok vydání: | 1998 |
| Předmět: |
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Western Molecular Sequence Data Neuromuscular Junction Biophysics Muscle Proteins Receptors Nicotinic Torpedo Biochemistry Neuromuscular junction law.invention Synapse Structural Biology law Genetics medicine Animals Amino Acid Sequence Molecular Biology Mitosis Acetylcholine receptor Rapsyn Base Sequence Myogenesis Chemistry Peripheral membrane protein RNA-Binding Proteins Cell Biology Cell biology medicine.anatomical_structure Nicotinic agonist nervous system Sam68 |
| Zdroj: | FEBS Letters. 437:29-33 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(98)01151-x |
| Popis: | Nicotinic acetylcholine receptors (nAChRs) are initially expressed diffusely on the surface of myotubes and, in response to neuronally derived factors, cluster at the endplate to a final concentration of approximately 10 000/μm2. The synaptic peripheral membrane protein rapsyn has been shown to mediate clustering of nAChRs in several systems. Here we describe the use of the yeast two-hybrid system to identify proteins that can interact with rapsyn. One of the clones we have identified is a Torpedo californica homolog of the Src-associated in mitosis protein (Sam68). We further show that Sam68, like rapsyn, is localized at the neuromuscular junction. |
| Databáze: | OpenAIRE |
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