A graphene modified carbon ionic liquid electrode for voltammetric analysis of the sequence of the Staphylococcus aureus nuc gene
| Autor: | Qin Wang, Guangjiu Li, Chen Wei, Xiuli Wang, Yongling Men, Xueliang Niu, Wei Sun |
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| Rok vydání: | 2018 |
| Předmět: |
Staphylococcus aureus
Inorganic chemistry Nanochemistry DNA Single-Stranded Ionic Liquids 02 engineering and technology Biosensing Techniques 010402 general chemistry Electrochemistry 01 natural sciences Analytical Chemistry law.invention chemistry.chemical_compound law Limit of Detection Electrodes Detection limit Base Sequence Graphene Nucleic Acid Hybridization Electrochemical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Methylene Blue chemistry Genes Bacterial Electrode Ionic liquid Graphite Differential pulse voltammetry 0210 nano-technology Methylene blue |
| Zdroj: | Mikrochimica acta. 185(3) |
| ISSN: | 1436-5073 |
| Popis: | The authors describe a voltammetric method for the detection of the nuc ssDNA sequence originating from Staphylococcus aureus by using a carbon ionic liquid electrode modified with electrodeposited three-dimensional graphene (3DGR). Probe ssDNA was electrostatically adsorbed on the modified electrode by a potentiostatic method. The porous structure and large surface area of 3DGR greatly increase the amount of immobilized probe ssDNA on the interface, which is beneficial for the reaction with target ssDNA. By using Methylene Blue (MB) as the electrochemical probe, the reduction peak current of MB (best measured at −0.30 V vs. SCE) can be used for detecting hybridization. The differential pulse voltammetric current of MB increases linearly in the 1.0 × 10−12 mol L−1 to 1.0 × 10−6 mol L−1 nuc concentration range, and the detection limit is 3.3 × 10−13 mol L−1 (at 3σ). The DNA sensor was successfully applied to the determination of the PCR product of the gene in pork. |
| Databáze: | OpenAIRE |
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