The distinct role of catalase and DNA repair systems in protection against hydrogen peroxide in Escherichiacoli
| Autor: | Yoshibumi Sato, Shuji Yonei, Reiko Yokota |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
inorganic chemicals
DNA Repair DNA repair DNA damage Operon Mutant Biophysics Biochemistry chemistry.chemical_compound Escherichia coli Hydrogen peroxide Molecular Biology biology Normal level Hydrogen Peroxide Cell Biology Catalase Bacteriophage lambda Molecular biology Highly sensitive chemistry Inactivation Metabolic biology.protein bacteria DNA Damage |
| Zdroj: | Biochemical and Biophysical Research Communications. 143:638-644 |
| ISSN: | 0006-291X |
| Popis: | The katEkatG mutant of E. coli , UM1, had no assayable catalase activities in the extract and showed increased (about 20 fold) sensitivity to killing by H2O2 when compared with its parental strain CSH7. The mutant strain was able to reactivate H2O2-damaged λ phage. On the other hand, recA and polA mutants were also highly sensitive to H2O2, but they had normal level of catalase activities. RecA derivatives of UM1 were much more sensitive to H2O2 than UM1 and recA strains. The induction of umu operon occurred in UM1 at lower ( 1 10 – 1 20 ) doses of H2O2 than in CSH7. From the results it is concluded that the lethal effect of H2O2 is due to DNA damage induced by it and that catalase and DNA repair systems have a distinct role in protection against H2O2 in E. coli . |
| Databáze: | OpenAIRE |
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