Natural variation in the promoter of rice calcineurin B-like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies
| Autor: | Chang-Xiang Zhu, Hao Zhang, Jianhua Zhang, Jianchang Yang, Lu Shi, Qijuan Hu, Hai-Yong Gu, Hui-Juan Yi, Feng-Zhu Wang, Li-Juan Xie, Nenghui Ye, Yinggao Liu, Tie Yuan Liu, Fu-Yuan Zhu, Shi Xiao, Xuan-Xuan Hou, Cao Yunying, Ze-Zhuo Su, Yi-Zhen Wu, Moxian Chen |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germination Plant Science Biology 01 natural sciences Japonica 03 medical and health sciences Species Specificity Stress Physiological parasitic diseases Genetics Cultivar Promoter Regions Genetic Plant Proteins Ecotype Oryza sativa Calcineurin fungi Flooding (psychology) food and beverages Genetic Variation Promoter Oryza Cell Biology biology.organism_classification Adaptation Physiological humanities Floods Horticulture 030104 developmental biology Upland and lowland Seeds Calcium Protein Kinases 010606 plant biology & botany |
| Zdroj: | The Plant journal : for cell and molecular biology. 94(4) |
| ISSN: | 1365-313X |
| Popis: | Rice (Oryza sativa L.) has two ecotypes, upland and lowland rice, that have been observed to show different tolerance levels under flooding stress. In this study, two rice cultivars, upland (Up221, flooding-intolerant) and lowland (Low88, flooding-tolerant), were initially used to study their molecular mechanisms in response to flooding germination. We observed that variations in the OsCBL10 promoter sequences in these two cultivars might contribute to this divergence in flooding tolerance. Further analysis using another eight rice cultivars revealed that the OsCBL10 promoter could be classified as either a flooding-tolerant type (T-type) or a flooding-intolerant type (I-type). The OsCBL10 T-type promoter only existed in japonica lowland cultivars, whereas the OsCBL10 I-type promoter existed in japonica upland, indica upland and indica lowland cultivars. Flooding-tolerant rice cultivars containing the OsCBL10 T-type promoter have shown lower Ca2+ flow and higher α-amylase activities in comparison to those in flooding-intolerant cultivars. Furthermore, the OsCBL10 overexpression lines were sensitive to both flooding and hypoxic treatments during rice germination with enhanced Ca2+ flow in comparison to wild-type. Subsequent findings also indicate that OsCBL10 may affect OsCIPK15 protein abundance and its downstream pathways. In summary, our results suggest that the adaptation to flooding stress during rice germination is associated with two different OsCBL10 promoters, which in turn affect OsCBL10 expression in different cultivars and negatively affect OsCIPK15 protein accumulation and its downstream cascade. |
| Databáze: | OpenAIRE |
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