Response to Infliximab Therapy in Ulcerative Colitis is Associated With Decreased Monocyte Activation, Reduced CCL2 Expression and Downregulation of Tenascin C
| Autor: | Kjell-Arne Ung, Anders Lasson, Stefan Isaksson, Maria K. Magnusson, Hans Strid, Antal Bajor, Lena Öhman |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Proteomics Chemokine Adolescent Biopsy Down-Regulation Inflammatory bowel disease Peripheral blood mononuclear cell Monocytes Young Adult Gastrointestinal Agents medicine Humans Intestinal Mucosa Chemokine CCL2 Aged biology Tumor Necrosis Factor-alpha business.industry Monocyte Tenascin C Gastroenterology Antibodies Monoclonal Tenascin DNA General Medicine Middle Aged Flow Cytometry medicine.disease Ulcerative colitis Infliximab Treatment Outcome medicine.anatomical_structure Immunology biology.protein Colitis Ulcerative Female Tumor necrosis factor alpha business Biomarkers Follow-Up Studies medicine.drug |
| Zdroj: | Journal of Crohn's and Colitis. 9:56-65 |
| ISSN: | 1876-4479 1873-9946 |
| DOI: | 10.1093/ecco-jcc/jju008 |
| Popis: | Background and aims: The cellular mechanisms leading to infliximab therapy response in patients with ulcerative colitis (UC) are incompletely known. We therefore investigated early effects of infliximab therapy on monocytes and associated chemokines linked to clinical therapy response in UC patients. Methods: Blood and biopsies were obtained from anti-TNF therapy-naive UC patients ( n = 43) before (baseline) and during induction therapy with infliximab. Therapy response was evaluated at Week 14. Expression of monocyte activation markers and levels of chemokines in serum and biopsies were determined. Quantitative proteomic analysis was performed in cultured mucosal biopsies, and obtained data was validated in serum. Results: In therapy responders, but not in non-responders, infliximab reduced blood monocyte expression of CD14 and CD86, 2 weeks after therapy commenced, relative to baseline. Serum CCL2 levels were decreased only among therapy responders at Week 2 and Week 14, relative to baseline. These data corresponded with lower levels of CD14, CD86 and CCL2 in intestinal tissue in responders as compared with non-responders at Week 14. Proteomic analysis of cultured biopsies showed that infliximab induced a reduction in Tenascin C that predicted downregulation of CCL2. Therapy responders, but not non-responders, had decreased serum Tenascin C levels at Week 2 and Week 14, relative to baseline. Conclusions: Infliximab therapy response in UC patients is associated with reduced monocyte activation and serum levels of CCL2 2 weeks after therapy commencement. In therapy responders, infliximab influenced Tenascin C, which might be a regulator of CCL2 expression and important for induction of the clinical therapy response. * Abbreviations: : UC : ulcerative colitis IBD : inflammatory bowel disease TNF : tumor necrosis factor ECM : extracellular matrix TNC : Tenascin C PBMC : peripheral blood mononuclear cell MS : mass spectrometry IPA : ingenuity pathway analysis IPA KB : Ingenuity Pathways Analysis Knowledge Base MAP : molecule activity predictor |
| Databáze: | OpenAIRE |
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