Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
| Autor: | Auinash Kalsotra, Zhigang Jin, Tristan Aguero, Sandip Chorghade, Mary Lou King, Jing Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Somatic cell TRANSLATION REGULATION Otras Ciencias Biológicas Xenopus Germline Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences 0302 clinical medicine Eukaryotic initiation factor Translational regulation purl.org/becyt/ford/1.6 [https] Molecular Biology Genetics biology Translation (biology) Embryo biology.organism_classification NANOS 030104 developmental biology DND1 XENOPUS GERMLINE DEVELOPMENT Transcription preinitiation complex 030217 neurology & neurosurgery CIENCIAS NATURALES Y EXACTAS Developmental Biology |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development. Fil: Agüero, Tristán Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. University of Miami; Estados Unidos Fil: Jin, Zhigang. University of Illinois at Urbana; Estados Unidos Fil: Chorghade, Sandip. University of Illinois at Urbana; Estados Unidos Fil: Kalsotra, Auinash. University of Illinois at Urbana; Estados Unidos Fil: King, Mary Lou. University of Miami; Estados Unidos Fil: Yang, Jing. University of Illinois at Urbana; Estados Unidos |
| Databáze: | OpenAIRE |
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