Genetic complexity of miscanthus cell wall composition and biomass quality for biofuels

Autor: Leonardo D. Gomez, Edouard Severing, Luisa M. Trindade, Claire Lessa Alvim Kamei, Andres F. Torres, Oene Dolstra, Chris Maliepaard, Tim van der Weijde, Richard G. F. Visser, Simon J. McQueen-Mason
Rok vydání: 2017
Předmět:
0106 biological sciences
Genetic Linkage
Biomass
Miscanthus sinensis
02 engineering and technology
Lignin
01 natural sciences
7. Clean energy
Laboratorium voor Plantenveredeling
Genetic map
Cell Wall
Biomass quality
0202 electrical engineering
electronic engineering
information engineering
2. Zero hunger
PBR Kwantitatieve aspecten
education.field_of_study
PBR Biobased Economy
biology
food and beverages
Miscanthus
PE&RC
Drug Combinations
Biofuel
PBR Bio-based Economy
Research Article
Biotechnology
Saccharification efficiency
Yield
lcsh:QH426-470
Genotype
lcsh:Biotechnology
020209 energy
Quantitative Trait Loci
Population
Lignocellulosic biomass
Poaceae
Synteny
complex mixtures
PBR Quantitative aspects of Plant Breeding
Species Specificity
Bioenergy
lcsh:TP248.13-248.65
Genetics
education
business.industry
Cell wall composition
Quantitative trait loci (QTL)
Genetic Variation
15. Life on land
biology.organism_classification
Sorghum
lcsh:Genetics
Plant Breeding
Agronomy
Biofuels
Pyrantel
EPS
business
Conversion efficiency
010606 plant biology & botany
Zdroj: BMC Genomics
BMC Genomics, 18
BMC Genomics, Vol 18, Iss 1, Pp 1-15 (2017)
BMC Genomics 18 (2017)
ISSN: 1471-2164
Popis: Background Miscanthus sinensis is a high yielding perennial grass species with great potential as a bioenergy feedstock. One of the challenges that currently impedes commercial cellulosic biofuel production is the technical difficulty to efficiently convert lignocellulosic biomass into biofuel. The development of feedstocks with better biomass quality will improve conversion efficiency and the sustainability of the value-chain. Progress in the genetic improvement of biomass quality may be substantially expedited by the development of genetic markers associated to quality traits, which can be used in a marker-assisted selection program. Results To this end, a mapping population was developed by crossing two parents of contrasting cell wall composition. The performance of 182 F1 offspring individuals along with the parents was evaluated in a field trial with a randomized block design with three replicates. Plants were phenotyped for cell wall composition and conversion efficiency characters in the second and third growth season after establishment. A new SNP-based genetic map for M. sinensis was built using a genotyping-by-sequencing (GBS) approach, which resulted in 464 short-sequence uniparental markers that formed 16 linkage groups in the male map and 17 linkage groups in the female map. A total of 86 QTLs for a variety of biomass quality characteristics were identified, 20 of which were detected in both growth seasons. Twenty QTLs were directly associated to different conversion efficiency characters. Marker sequences were aligned to the sorghum reference genome to facilitate cross-species comparisons. Analyses revealed that for some traits previously identified QTLs in sorghum occurred in homologous regions on the same chromosome. Conclusion In this work we report for the first time the genetic mapping of cell wall composition and bioconversion traits in the bioenergy crop miscanthus. These results are a first step towards the development of marker-assisted selection programs in miscanthus to improve biomass quality and facilitate its use as feedstock for biofuel production. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3802-7) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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